SCC-9 tongue squamous cell carcinoma cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

SCC-9 tongue squamous cell carcinoma cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue tongue
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease squamous cell carcinoma
Age 25 years
Gender male
Clinical Data
male
25 years
Genes Expressed
epidermal keratins; low levels of involucrin
Cellular Products
epidermal keratins; low levels of involucrin
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
Growth of SCC-9 is enhanced by using a feeder layer of Mitomycin C-treated STO cells (ATCC 56-X.2).
The cells do not grow well in semi-solid medium.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8
TH01: 8,9
TPOX: 9,11
vWA: 17
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net