AVG-16猪可逆永生化原代颗粒细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥49865
- 货 号:AVG-16猪可逆永生化原代颗粒细胞株
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- 联系人:Dr.Xu, Biovector NTCC Inc.
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邮件:Biovector@163.com
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地址:北京
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AVG-16猪可逆永生化原代颗粒细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Origin
Porcine Reversibly Immortalised Primary Granulosa Cells
Cell Line Description
AVG-16 consists of reversibly immortalised primary granulosa cells. Primary cultures of pig granulosa cells have been cultured in the presence of the media supplement Ovagrow-S2A resulting in the establishment of an immortalised porcine primary granulosa cell line. Ovagrow-S2A, produced by Aivogen, comprises a naturally derived complex of growth promoting compounds, carrier proteins, and factors. These cells are not contact inhibited. The cells are conditionally immortalised; cell death will occur in the absence of OvagrowS2A and/or fibroblast growth factor-basic (bFGF).
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1% NEAA + 2.5ng/ml Fibroblast Growth Factor-Basic (bFGF) + 4% OvagrowS2A
Subculture Routine
Split subconfluent cultures (70-80%) 1:5 to 1:10, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Saturation density: 1.5x100,000 cells/cm2.
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
Cell Line Origin
Porcine Reversibly Immortalised Primary Granulosa Cells
Cell Line Description
AVG-16 consists of reversibly immortalised primary granulosa cells. Primary cultures of pig granulosa cells have been cultured in the presence of the media supplement Ovagrow-S2A resulting in the establishment of an immortalised porcine primary granulosa cell line. Ovagrow-S2A, produced by Aivogen, comprises a naturally derived complex of growth promoting compounds, carrier proteins, and factors. These cells are not contact inhibited. The cells are conditionally immortalised; cell death will occur in the absence of OvagrowS2A and/or fibroblast growth factor-basic (bFGF).
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 1% NEAA + 2.5ng/ml Fibroblast Growth Factor-Basic (bFGF) + 4% OvagrowS2A
Subculture Routine
Split subconfluent cultures (70-80%) 1:5 to 1:10, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Saturation density: 1.5x100,000 cells/cm2.
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
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