HUMAN BREAST TUMOR-ASSOCIATED ENDOTHELIAL CELLS hc-6020 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

HUMAN BREAST TUMOR-ASSOCIATED ENDOTHELIAL CELLS hc-6020 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Human Breast Tumor-Associated Endothelial Cells

Catalog No. HC-6020

Suggested Medium: Complete Human Endothelial Cell Medium– 500ML

Catalog No. H1168

Product Description

Human Breast Tumor-Associated Endothelial Cells from Cell Biologics are isolated from human breast tumor tissue. Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.

Product Testing

Human Breast Tumor-Associated Endothelial Cells from Cell Biologics display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R&D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 2-4 passages under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Human Breast Tumor-Associated Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net