TFK-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

TFK-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
TFK-1 cell line
Cat No.: NTCC-DACC344

Cell line:
TFK-1
Species:
human (Homo sapiens)
Cell type:
bile duct carcinoma
Origin:
established from the surgical specimen extracted from a 63-year-old Japanese man with extrahepatic bile duct carcinoma; described in the literature to express c-erbB-2 protein
Reference(s):
Saijyo, S., Kudo, T., Suzuki, M., Katayose, Y., Shinoda, M., Muto, T., Fukuhara, K., Suzuki, T., Matsuno, S. (1995). Establishment of a new extrahepatic bile duct carcinoma cell line, TFK-1. Tohoku Journal of Experimental Medicine 177 (1): 61-71 .

Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
epithelial cells growing as monolayers;
image;

Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (for 10-15 min)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 10-30 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact biovector@163.com
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed originally as human with IEF of AST, MDH, NP
Cytogenetics:
human flat-moded hypertriploid karyotype with 20% polyploidy - 72(64-81)<3n>XY/XYY, +1, +3, +5, +5, +6, -9, +11, -13, -16, -18, +20, +2mar, del(1)(q21), del(1)(p11), i(1q), del(3)(p12p21)x2, add(5)(q35), i(5p)x2, inv(7)(q11q35), del(11)(p11)x1-2, i(15p), i(17p) - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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