YBLW4， YBLW5，YBLW6，YBLW7，YBLW8 YBLW9 strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

YBLW4， YBLW5，YBLW6，YBLW7，YBLW8
YBLW9 strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
S. cerevisiae genotypes
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Contents
1 Common yeast strains
1.1 S288C
1.2 AB1380
1.3 J57D
1.4 W303-1a
1.5 VL6-48N
1.6 YPH499
1.7 YPH500
2 Window Strains
2.1 YBLW1A
2.2 YBLW2
2.3 YBLW3
2.4 YBLW4
2.5 YBLW5
2.6 YBLW6
2.7 YBLW7
2.8 YBLW8
2.9 YBLW9
3 References
Common yeast strains
van Dijken00 describes the characteristics of many common laboratory strains.

S288C
MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

gal2-; does not use galactose anaerobically
does not form pseudohyphae
ATCC: 204508
Mortimer86

AB1380
MATa ura3-52 trp1-289 lys2-1 ade2-1 can1-100 his5 ρ+ ψ+

ATCC 204682, 20843, 201447
AB1380 is MATa not MATα in contradiction to the article in Science 236: 806-812, 1987
Burke87

J57D
MATa ura3-52 trp1 ade2-101 can1-100 leu2-3,112 his3-2,15

used in Haldi96 for the mouse genome project
from Vladimir Larionov
Zhong98 claims MATα and his3-6
several times better transformation efficiency than AB1380

W303-1a
MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ybp1-1

Open Biosystems: YSC1058
Rothstein notes
Veal03

VL6-48N
MATα trp1-Δ1 ura3-Δ1 ade2-101 his3-Δ200 lys2 met14 cir°

high transformation efficiency strain for spheroplast YAC construction
Vladimir Larionov papers on TAR (transformation associated recombination)
generated from VL6-48 by substitution of the KanMX cassette into ura3-52
Construction: Kouprina98 (from VL6-48); Larionov96 (from YPH857, Sikorski89, Larionov94)

YPH499
MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

derived from S288C
contains deletions of most selectable markers
retains the promoter for the trp gene, thus homology with that plasmid sequence
gal2-; does not use galactose anaerobically
ATCC:204679
Sikorski89, Sobel99

YPH500
MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his1-Δ200 leu2-Δ1

derived from S288C
contains deletions of most selectable markers
retains the promoter for the trp gene, thus homology with that plasmid sequence
gal2-; does not use galactose anaerobically
ATCC:204680
Sikorski89, Sobel99

Window Strains
Strains are derivative of YPH925
Strains have gaps in the chromosome size range to allow gel extraction of YACs with specific sizes. See Hamer95.
PFG conditions are BioRad Chef 1% SeaPlaque GTG agarose at 6 V/cm 0.5x TBE 12 C
YACs can be transfered to these strains by kar1- mating, see Hugerat94 and Spencer94.

YBLW1A
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 cys3

Window 150-280 Kbp
PFG 15-25s, 24 hrs
Genotype is listed for YBLW1; YBLW1A may have differences, but is the strain we have.

YBLW2
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

Window 250-450 Kbp
PFG 20-35s, 24 hrs

YBLW3
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met10 thr4

Window 310-590 Kbp
PFG 35-60s, 24 hrs

YBLW4
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 thr1

Window 450-680 Kbp
PFG 50-70s, 24 hrs

YBLW5
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15 met14

Window 590-755 Kbp
PFG 50-80s, 36 hrs

YBLW6
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

Window 680-950 Kbp
PFG 60-100s, 36 hrs

YBLW7
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

Window 810-1120 Kbp
PFG 70-120s, 48 hrs

YBLW8
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

Window 985-1640 Kbp
PFG 80-130s, 48 hrs

YBLW9
MATα leu2-Δ1 trp1-Δ63 ura3-52 ade2-101 his3-Δ200 lys2-801 cyh2R kar1-Δ15

Window 1140-2000 Kbp (upper range varies due to the presence of ribosomal repeats in chromosome XII)
PFG 100-160s, 48 hrs

References
Mortimer RK and Johnston JR. Genealogy of principal strains of the yeast genetic stock center. Genetics. 1986 May;113(1):35-43. PubMed ID:3519363 | HubMed [Moritmer86]
Burke DT, Carle GF, and Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806-12. DOI:10.1126/science.3033825 | PubMed ID:3033825 | HubMed [Burke87]
Sikorski RS and Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19-27. PubMed ID:2659436 | HubMed [Sikorski89]
Hugerat Y, Spencer F, Zenvirth D, and Simchen G. A versatile method for efficient YAC transfer between any two strains. Genomics. 1994 Jul 1;22(1):108-17. DOI:10.1006/geno.1994.1351 | PubMed ID:7959756 | HubMed [Hugerat94]
Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, and Hieter P. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics. 1994 Jul 1;22(1):118-26. DOI:10.1006/geno.1994.1352 | PubMed ID:7959757 | HubMed [Spencer94]
Larionov V, Kouprina N, Nikolaishvili N, and Resnick MA. Recombination during transformation as a source of chimeric mammalian artificial chromosomes in yeast (YACs). Nucleic Acids Res. 1994 Oct 11;22(20):4154-62. DOI:10.1093/nar/22.20.4154 | PubMed ID:7937141 | HubMed [Larionov94]
Hamer L, Johnston M, and Green ED. Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations. Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11706-10. DOI:10.1073/pnas.92.25.11706 | PubMed ID:8524833 | HubMed [Hamer95]
Kouprina N, Annab L, Graves J, Afshari C, Barrett JC, Resnick MA, and Larionov V. Functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3' end target sequence. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4469-74. DOI:10.1073/pnas.95.8.4469 | PubMed ID:9539761 | HubMed [Kouprina98]
Sobel SG and Wolin SL. Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes. Mol Biol Cell. 1999 Nov;10(11):3849-62. DOI:10.1091/mbc.10.11.3849 | PubMed ID:10564276 | HubMed [Sobel99]
van Dijken JP, Bauer J, Brambilla L, Duboc P, Francois JM, Gancedo C, Giuseppin ML, Heijnen JJ, Hoare M, Lange HC, Madden EA, Niederberger P, Nielsen J, Parrou JL, Petit T, Porro D, Reuss M, van Riel N, Rizzi M, Steensma HY, Verrips CT, Vindeløv J, and Pronk JT. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb Technol. 2000 Jun 1;26(9-10):706-714. DOI:10.1016/s0141-0229(00)00162-9 | PubMed ID:10862876 | HubMed [VanDijken00]
Veal EA, Ross SJ, Malakasi P, Peacock E, and Morgan BA. Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor. J Biol Chem. 2003 Aug 15;278(33):30896-904. DOI:10.1074/jbc.M303542200 | PubMed ID:12743123 | HubMed [Veal03]
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