VR-764 Murine hepatitis virus鼠肝炎病毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心

VR-764 Murine hepatitis virus鼠肝炎病毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Murine hepatitis virus (VR-764）
Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2 / Product Format: frozen
Classification Nidovirales, Coronaviridae, Coronavirus,Group 2
Agent Murine hepatitis virus
Strain MHV-A59
Common Name Murine hepatitis virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched position
Comments
The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounding and sloughing, attached and floating syncytial debris
Growth Medium NCTC 135 medium, 90%; horse serum, 10%
Recommended Host
NCTC clone 1469 cells (ATCC CCL-9.1)
Recommended Host: NCTC 1469 (ATCC CCL-9.1)

Growth Conditions
Incubation: 18 to 24 hours at 37°C, a 5%CO2 in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Temperature: 37°C
References
Piczak CV, et al. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29-51, 1961. PubMed: 13766009

rounding and sloughing with attached and floating syncytial debris
Cryopreservation
Storage Temperature: -70°C or colder
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, rounding and sloughing, attached and floating syncytial debris
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