CAS9 Purified Streptococcus pyogen Protein纯化蛋白 BioVector NTCC质粒载体菌种细胞基因保藏中心

CAS9 Purified Streptococcus pyogen Protein纯化蛋白 BioVector NTCC质粒载体菌种细胞基因保藏中心
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli, 500 pmol
Species: Streptococcus pyogen Expression Host: E.coli Expression cDNA Clone or AA Sequence 1 Tag: N-terminal HIS Predicted MW: 165.0 kDa Purity: > 95% as determined by SDS-PAGE and Coomassie blue staining Concentration: 20 uM Buffer: 10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol. Endotoxin: < 0.5 EU per 1 ?g of the protein by the LAL BioActivity: In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease. The input DNA is XmnI-linearized vector was cleaved by with a synthetic sgRNA.
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