NCI-H520 cell line人肺鳞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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NCI-H520 cell line人肺鳞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
NCI-H520 [H520] (ATCC® HTB-182™)Organism: Homo sapiens, human / Tissue: lung / Disease: squamous cell carcinomaOrganism Homo sapiens, humanTissue lungProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease squamous cell carcinomaGender maleApplications This cell line is a suitable transfection host.Karyotype This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.Derivation The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.Clinical Data maleTumorigenic YesEffects Yes, in nude mice inoculated subcutaneously with 10(7) cells(Tumors developed within 21 days at 100% frequency (5/5).)Comments The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.The line can be cloned in soft agar (with or without serum).Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: 2 to 3 times per weekCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CSTR Profile Amelogenin: XCSF1PO: 10D13S317: 10,11D16S539: 8,13D5S818: 12,13D7S820: 8,12TH01: 10TPOX: 8vWA: 18,19Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 2Me-2, 0PGM1, 1PGM3, 1Population Doubling Time 61 hrs in medium with serum; 25 to 32 hrs in serum-free mediumDeposited As Homo sapiensReferences Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
Supplier来源:BioVector NTCC Inc.
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NCI-H520 [H520] (ATCC® HTB-182™)Organism: Homo sapiens, human / Tissue: lung / Disease: squamous cell carcinomaOrganism Homo sapiens, humanTissue lungProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease squamous cell carcinomaGender maleApplications This cell line is a suitable transfection host.Karyotype This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.Derivation The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.Clinical Data maleTumorigenic YesEffects Yes, in nude mice inoculated subcutaneously with 10(7) cells(Tumors developed within 21 days at 100% frequency (5/5).)Comments The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.The line can be cloned in soft agar (with or without serum).Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Subculturing Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: 2 to 3 times per weekCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CSTR Profile Amelogenin: XCSF1PO: 10D13S317: 10,11D16S539: 8,13D5S818: 12,13D7S820: 8,12TH01: 10TPOX: 8vWA: 18,19Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 2Me-2, 0PGM1, 1PGM3, 1Population Doubling Time 61 hrs in medium with serum; 25 to 32 hrs in serum-free mediumDeposited As Homo sapiensReferences Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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