NCI-H226 cell line人肺鳞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：NCI-H226 cell line人肺鳞癌细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
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地址：北京
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NCI-H226 cell line人肺鳞癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
NCI-H226 [H226] (ATCC® CRL-5826™)Organism: Homo sapiens, human / Tissue: lung; derived from metastatic site: pleural effusion / Disease: squamous cell carcinoma; mesotheliomaOrganism Homo sapiens, humanTissue lung; derived from metastatic site: pleural effusionProduct Format frozenMorphology EpithelialCulture Properties adherentBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease squamous cell carcinoma; mesotheliomaGender maleKaryotype modal number = 47; range = 32-88; del(p25)Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: 1:4 to 1:8 is recommendedMedium Renewal: Every 2 to3 daysCryopreservation Freeze medium: Culture medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CSTR Profile Amelogenin: X,YCSF1PO: 10,11D13S317: 13,14D16S539: 9,12D5S818: 11,12D7S820: 8,10TH01: 8,9.3TPOX: 8,11vWA: 17
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
NCI-H226 [H226] (ATCC® CRL-5826™)Organism: Homo sapiens, human / Tissue: lung; derived from metastatic site: pleural effusion / Disease: squamous cell carcinoma; mesotheliomaOrganism Homo sapiens, humanTissue lung; derived from metastatic site: pleural effusionProduct Format frozenMorphology EpithelialCulture Properties adherentBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease squamous cell carcinoma; mesotheliomaGender maleKaryotype modal number = 47; range = 32-88; del(p25)Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: 1:4 to 1:8 is recommendedMedium Renewal: Every 2 to3 daysCryopreservation Freeze medium: Culture medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CSTR Profile Amelogenin: X,YCSF1PO: 10,11D13S317: 13,14D16S539: 9,12D5S818: 11,12D7S820: 8,10TH01: 8,9.3TPOX: 8,11vWA: 17
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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