首页 » pVSVdeltaG-GFP病毒包装荧光报告质粒载体pVSV-ΔG-GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pVSVdeltaG-GFP病毒包装荧光报告质粒载体pVSV-ΔG-GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98635
  • 货  号:pVSVdeltaG-GFP病毒包装荧光报告质粒载体pVSV-ΔG-GFP
  • 产  地:北京
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pVSVdeltaG-GFP病毒包装荧光报告质粒载体pVSV-ΔG-GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

The plasmid pVSV-ΔG-GFP encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with GFP. This plasmid is used together with plasmids encoding the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) to recovery VSV-G pseudotyped ΔG-GFP virus as described in [1]. The antigenomic RNA of ΔG-GFP is expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain the hepatitis delta ribozyme used to generate a precise 3’ end of the VSV antigenomic RNA and a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [2, 3].

Recombinant vesicular stomatitis viru-ΔG (rVSV-ΔG) has been used to produce VSV pseudotypes containing the envelope glycoproteins of heterologous viruses including viruses that require high-level containment. Since the infectivity of rVSV-ΔG is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.
Product Type: Plasmid
Alternative Name(s): antigenomic-sense (or positive-sense) RNA of vesicular stomatitis virus (VSV) ΔG-GFP
Gene/insert name: ΔG-GFP
Antibiotic Resistance: Ampicillin
Fusion Tag(s): GFP
Grow in E. coli at 37 C: Yes
Cloning Site 5': 5' VSV sequence joined directly to T7 promoter
Cloning Site 3': 3' VSV sequence joined directly to HDV ribozyme
Insert Size: 10,354 bp
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Shipped: Ambient temperature


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