VSV-ΔG-Luciferase Plasmid Expression Vector质粒载体pVSV-deltaG-Luc BioVector NTCC质粒载体菌种细胞基因保藏中心

VSV-ΔG-Luciferase Plasmid Expression Vector质粒载体pVSV-deltaG-Luc BioVector NTCC质粒载体菌种细胞基因保藏中心

The plasmid pVSV-ΔG-Luciferase encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with firefly luciferase. This plasmid is used together with plasmids encoding the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) to recovery VSV-G pseudotyped ΔG- Luciferase virus as described in [1]. The antigenomic RNA of ΔG-Luciferase is expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain the hepatitis delta ribozyme used to generate a precise 3’ end of the VSV antigenomic RNA and a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+ [2, 3].

Recombinant vesicular stomatitis virus-ΔG (rVSV-ΔG) has been used to produce VSV pseudotypes containing the envelope glycoproteins of heterologous viruses including viruses that require high-level containment. Since the infectivity of rVSV-ΔG is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.
Product Type: Plasmid
Alternative Name(s): antigenomic-sense (or positive-sense) RNA of vesicular stomatitis virus (VSV) ΔG-Luciferase
Gene/insert name: ΔG-Luciferase
Antibiotic Resistance: Ampicillin
Fusion Tag(s): luciferase
Grow in E. coli at 37 C: Yes
Cloning Site 5': 5' VSV sequence joined directly to T7 promoter
Cloning Site 3': 3' VSV sequence joined directly to HDV ribozyme
Insert Size: 11,352 bp
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Shipped: Ambient temperature

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