pVSV-ΔG plasmid vector质粒载体VSV-deltaG BioVector NTCC质粒载体菌种细胞基因保藏中心

pVSV-ΔG plasmid vector质粒载体VSV-deltaG BioVector NTCC质粒载体菌种细胞基因保藏中心

The plasmid pVSV-ΔG encodes the antigenomic-sense (or positive-sense) RNA of a replication-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been deleted and replaced with a multiple cloning site containing sequences for the following restriction enzymes (5’-MluI-KpnI-XhoI-XmaI-EagI-SphI-NheI-3’), which are all unique in the plasmid.
pVSV-ΔG can be used to insert a heterologous gene into the MCS for expression from the VSV genome. For example expression of a heterologous glycoprotein, a cytokine, or a reporter such as GFP or luciferase. Such vectors would be useful for vaccine studies, for use as a reporter virus, or in identification of host entry factors.
This plasmid is used together with plasmids encoding the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) to recovery VSV-G pseudotyped ΔG-virus. The antigenomic RNA of ΔG-PL VSV is expressed from the bacteriophage T7 promoter in pBluescript, which has been further modified to contain the hepatitis delta ribozyme used to generate a precise 3’ end of the VSV antigenomic RNA and a T7 terminator sequence cloned between the SacII and SacI restriction sites in pBS-SK+.

Product Type: Plasmid
Antibiotic Resistance: Ampicillin
Fusion Tag(s): None
Amount: 10 ul aliquot of plasmid in shipping vial
Grow in E. coli at 37 C: Yes
Cloning Site 5': 5' VSV sequence joined directly to T7 promoter
Cloning Site 3': 3' VSV sequence joined directly to HDV ribozyme
Insert Size: 12,733 bp
Vector Backbone and Size: pBS-SK-ΦT, 3105 bp
High or low copy: High
Shipped: Ambient temperature


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