NCI-H1048 [H1048] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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NCI-H1048 [H1048] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, humanTissue lung; derived from metastatic site: pleural effusionProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Disease carcinoma; small cell lung cancerGender femaleKaryotype 3p is normalImages Derivation The line was established in April 1985 from pleural effusion metastasis of lung.Clinical Data femaleThe tissue donor was a non-smoker.Complete Growth Medium HITES medium supplemented with 5% fetal bovine serumThe base medium for this cell line is DMEM:F12 Medium. To make the complete growth medium,add the following components to the base medium0.005 mg/ml Insulin0.01 mg/ml Transferrin30nM Sodium selenite (final conc.)10 nM Hydrocortisone (final conc.)10 nM beta-estradiol (final conc.)extra 2mM L-glutamine (for final conc. of 4.5 mM)5% fetal bovine serum (final conc.)Subculturing Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 x 104 to 2 0 x 104 viable cells/cm2 is recommended.Subcultivation Ratio: 1:3 to 1:8Incubate cultures at 37°C. Subculture when cell concentration is between 9 x 104 and 1.7 x 105 cells/cm2.Cryopreservation Culture medium, 95%; DMSO, 5%Year of Origin 1985References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Supplier来源:BioVector NTCC Inc.
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Organism Homo sapiens, humanTissue lung; derived from metastatic site: pleural effusionProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Disease carcinoma; small cell lung cancerGender femaleKaryotype 3p is normalImages Derivation The line was established in April 1985 from pleural effusion metastasis of lung.Clinical Data femaleThe tissue donor was a non-smoker.Complete Growth Medium HITES medium supplemented with 5% fetal bovine serumThe base medium for this cell line is DMEM:F12 Medium. To make the complete growth medium,add the following components to the base medium0.005 mg/ml Insulin0.01 mg/ml Transferrin30nM Sodium selenite (final conc.)10 nM Hydrocortisone (final conc.)10 nM beta-estradiol (final conc.)extra 2mM L-glutamine (for final conc. of 4.5 mM)5% fetal bovine serum (final conc.)Subculturing Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 x 104 to 2 0 x 104 viable cells/cm2 is recommended.Subcultivation Ratio: 1:3 to 1:8Incubate cultures at 37°C. Subculture when cell concentration is between 9 x 104 and 1.7 x 105 cells/cm2.Cryopreservation Culture medium, 95%; DMSO, 5%Year of Origin 1985References NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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