U87/TMZ人神经胶质瘤细胞替莫唑胺耐药细胞株TMZ-R U87 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥598635
- 货 号：U87/TMZ人神经胶质瘤细胞替莫唑胺耐药细胞株TMZ-R U87
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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U87/TMZ人神经胶质瘤细胞替莫唑胺耐药细胞株TMZ-R U87 BioVector NTCC质粒载体菌种细胞基因保藏中心
The TMZ-R U87 glioblastoma cell line was established in vitro and in vivo. TMZ-R U87 cells showed a more invasive activity and a shorter survival time in vivo. Gene expression analysis using DNA microarray and quantitative PCR (qPCR) demonstrated that YKL-40, MAGEC1 and MGMT mRNA expression was upregulated 100-, 83- and 6-fold, respectively in the TMZ-R U87 cell line. Western blot analysis and qPCR demonstrated that STAT3 phosphorylation, STAT3 target genes and stem cell and mesenchymal marker genes were upregulated to a greater extent in the TMZ-resistant cell line. Notably, short hairpin (sh)RNA-based inhibition against the YKL-40 gene resulted in moderate growth inhibition in the resistant cells in vitro and in vivo. Additionally, YKL-40 gene inhibition exhibited significant suppression of the invasive activity and particularly partially restored the sensitivity to TMZ.The IC50 values of the U87 and TMZ-R U87 cells were 45 and >500 µM, respectively.Morphologically, TMZ-R U87 cells did not show a tendency for aggregation when they reached subconfluency. The activation (phosphorylation) of STAT3 and upregulation of MGMT were identified in the TMZ-R U87 cell line compared with the U87 cell line using WB analysis. Additionally, the TMZ-R U87 cell line exhibited a greater invasive activity compared with the parental U87 cell line.TMZ-R U87 cell-transplanted mice showed significant resistance to TMZ and a shorter survival time in vivo.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The TMZ-R U87 glioblastoma cell line was established in vitro and in vivo. TMZ-R U87 cells showed a more invasive activity and a shorter survival time in vivo. Gene expression analysis using DNA microarray and quantitative PCR (qPCR) demonstrated that YKL-40, MAGEC1 and MGMT mRNA expression was upregulated 100-, 83- and 6-fold, respectively in the TMZ-R U87 cell line. Western blot analysis and qPCR demonstrated that STAT3 phosphorylation, STAT3 target genes and stem cell and mesenchymal marker genes were upregulated to a greater extent in the TMZ-resistant cell line. Notably, short hairpin (sh)RNA-based inhibition against the YKL-40 gene resulted in moderate growth inhibition in the resistant cells in vitro and in vivo. Additionally, YKL-40 gene inhibition exhibited significant suppression of the invasive activity and particularly partially restored the sensitivity to TMZ.The IC50 values of the U87 and TMZ-R U87 cells were 45 and >500 µM, respectively.Morphologically, TMZ-R U87 cells did not show a tendency for aggregation when they reached subconfluency. The activation (phosphorylation) of STAT3 and upregulation of MGMT were identified in the TMZ-R U87 cell line compared with the U87 cell line using WB analysis. Additionally, the TMZ-R U87 cell line exhibited a greater invasive activity compared with the parental U87 cell line.TMZ-R U87 cell-transplanted mice showed significant resistance to TMZ and a shorter survival time in vivo.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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