CHO DG44 Cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

CHO DG44 Cell line细胞株

The gene amplification techniques have been used for production of glycoprotein in recombinant mammalian cell cultures. In these techniques, the dihydrofolate reductase (dhfr) gene amplification system in Chinese Hamster Ovary (CHO) cell line CHO DG44 is most available. CHO DG44 cell line is dihydrofolate reductase negative (dhfr-).
For almost 30 years, Chinese hamster ovary (CHO) cells have been extensively used for the production of recombinant proteins and can be considered the workhorse of modern glycoprotein production.
Not only can these cells express recombinant proteins with post-translational modifications compatible to humans, they do so by secreting the protein of interest into their environment and grow in serum-free suspension culture which makes them ideal for large scale high-titre cultures in the industry.
CHO cells lacking DHFR are the most widely used CHO cells for the industrial production of biopharmaceuticals.
The obtained CHO DG44 cell line has been subsequently adapted to serum-free, chemically defined cell culture media and suspension growth, which has resulted in a host cell line that is metabolically optimized for stable growth over longer periods of time to meet the large scale requirements for protein expression and genetic stability. A fully characterized cell bank of the host cell line has been prepared with full cell line history.

Organism Type Hamster
Tissues Ovary
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions,Transient Transfection

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