L6-GLUT4myc Rat Myoblast Cell Line BioVector NTCC质粒载体菌种细胞基因保藏中心

L6-GLUT4myc Rat Myoblast Cell Line

Immortalized rat skeletal muscle cells (L6) over-expresses GLUT4 with a myc-epitope, which is useful for measuring GLUT4 translocation. Highlights: L6 myoblast cell line that stably expresses GLUT4 protein containing a fourteen amino acid epitope of human c-myc within its first exofacial loopOnly cellular model available to study glucose uptake and GLUT4 translocation without permeabilizing or fractionating the cellsUseful for novel anti-diabetic compound screeningAmendable for transient transfection by plasmid-based gene transfer and viral infection protocols (retrovirus and adenovirus) Insulin stimulation of glucose transport in skeletal muscle results mainly due to translocation of the glucose transporter GLUT4 to the cell surface. L6 cells, originally derived from rat skeletal muscle, propagate as mononucleated myoblasts but can differentiate into multinucleated primary myotubes. The myotubes express several proteins typical of skeletal muscle including the GLUT4 glucose transporter. Insulin stimulates glucose uptake with high sensitivity and maximal responsiveness only in differentiated L6 myotubes and GLUT4 expression parallels the acquisition of these characteristics as the L6 cells differentiate. These features of L6 myotubes are important since GLUT4 is responsible for insulin-dependent glucose uptake in mature skeletal muscle.
Product Type: Cell Line Name: L6-GLUT4myc Cell Type: Skeletal muscle Accession ID: CVCL_0P25 Organism: Rat Morphology: Myoblast/Myotube Source: Quadriceps Biosafety Level: II Growth Conditions: MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS, 1% Antimycotic +5 ug/ml blasticidin Subculturing: 2-3 days Cryopreservation: MEM-α +10% FBS+ 10% DMSO Comments: The myc-epitope in the GLUT4myc has the amino acid sequence of AEEQKLISEEDLLK

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