MMMbz Blasticidin/Zeocin Resistant Cell Line BioVector NTCC质粒载体菌种细胞基因保藏中心

MMMbz Blasticidin/Zeocin Resistant Cell Line

The MMM cell line is very effective in supporting the growth of human embryonic stem (hES) cells. This line has been made resistant to blasticidin and zeocin (MMMbz), which are the most efficient drugs available for selection of hES cells transfected with exogenous genes. Highlights: Indefinite lifespan (immortalized) and rapid growth rateBetter support of hES cells, compared to MEFsExcellent ability to support growth of difficult-to-grow hES lines, with maintenance of the pluripotential stateResistance to blasticidin and zeocin makes it possible to select, by drug treatment, for hES cells into which exogenous genes have been introduced, without killing the supporting MMM Human embryonic stem (ES) cells are usually co-cultivated with supporting cells consisting of short-term cultures of fibroblasts (not an immortalized line) in a medium lacking serum. The immortalized MMMbz cell line supports the multiplication of H9 cells better than the 3T3 line. It supports the growth of H9 cells as well as do available short-term fibroblast cultures, but maintains more effectively the stem cell character of the H9 cells, judging by their better retention of Oct4. The cells are also resistant to blasticidin and zeocin, the most efficient antibiotics for selection of stable transformants.
Product Type: Cell Line Name: MMMbz Cell Type: Immortalized embryonic mouse fibroblasts with drug resistance to blasticidin and zeocin. Organism: Mouse Accession ID: CVCL_S558 Morphology: Fibroblastic Source: 12-13 day mouse embryos Biosafety Level: BL1 Subculturing: Subculture the cells once they reach 60-80% confluency. NOTE: It is very important not to let the cells get confluent or their ability to support hES cells will be diminished. Growth Conditions: Cells grow in 10% CO2 at 37C in a humidified incubator. Media: DMEM supplemented with 10% bovine calf serum. Split the cells at a density of ~ 3.3 x 103cells/cm2. Feed every 2 to 3 days with DME supplemented with 10% bovine calf serum. DO NOT use fetal bovine serum (Recommended serum: GE Healthcare Iron-Supplemented BVN CLF 500ml; Cat.SH30072.03). Cryopreservation: DMEM with 10% bovine calf serum containing 10% sterile DMSO

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