Murine Osteoblast to Osteocyte-like Cell Line MLO-A5 BioVector NTCC质粒载体菌种细胞基因保藏中心

Murine Osteoblast to Osteocyte-like Cell Line MLO-A5

MLO-A5 cell line is a model for the osteoblast to osteocyte differentiation process, the mineralization process, and the effects of mechanical loading on biomineralization. Highlights: Derived from a transgenic mouse in which the immortalizing T-antigen was expressed under control of the osteocalcin promoterExpress markers of the late osteoblast such as extremely high alkaline phosphatase, bone sialoprotein, PTH type 1 receptors, and osteocalcinRapidly mineralize in sheets, not nodulesForms a “honeycomb”-like mineralized matrix within 7–9 days of culture Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line MLO-A5 make it easier to study osteocyte function.
Product Type: Cell Line Name: MLO-A5 Cell Type: Postosteoblast/preosteocyte Accession ID: CVCL_0P24 Organism: Mouse Morphology: Adherent osteoblast-like cell; honeycomb-like mineralized matrix within 7-9 days of culture Biosafety Level: I Growth Conditions: Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 5% FBS and 5% CS, both heat-inactivated; penicillin-streptomycin at 100U/ml-100ug/ml Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; approximately 100µg/ml Ascorbic Acid and 4mM β-glycerophosphate (see Comments). Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Subculturing: Maintain stock cultures in proliferation medium under subconfluent conditions on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:10 to 1:20 dilution using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81) Cryopreservation: 60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6 cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81)

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