Murine Osteocyte-like Cell Line MLO-Y4 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：Murine Osteocyte-like Cell Line MLO-Y4
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Murine Osteocyte-like Cell Line MLO-Y4
MLO-Y4 cell line is useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways. Highlights: Derived from a transgenic mouse in which the immortalizing T-antigen was expressed under control of the osteocalcin promoterHigh expression of osteocalcinHigh expression of connexin 43 and the antigen E11Retain a dendritic morphology, similar to that observed in primary osteocyte Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line MLO-Y4 makes it easier to study osteocyte function.Product Type: Cell Line Name: MLO-Y4 Cell Type: Osteocyte Organism: Mouse (female) Accession ID: CVCL_M098 Morphology: Dendritic morphology Biosafety Level: I Subculturing: Maintain stock cultures in complete culture medium at ~60-75% confluence on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:5 dilution (~1:7maximum) using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81 Growth Conditions: alpha-MEM, containing L-glutamine and deoxyribonucleosides, with penicillin/streptomycin at 100 U/ml and 2.5% FBS and 2.5% CS; both heat inactivated; Grown on dishes coated with [0.15 mg/ml] rat tail type 1 collagen (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81). Cryopreservation: 60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81) Comments: When first starting these cells, centrifuge at approx. 1,000 rpm, for 5–10 min. Aspirate media, gently resuspend and plate the cell pellet in media containing 5% FBS and 5% CS. This higher amount of serum, 10% total, is useful to give the cells an extra boost. The next day, check the viability of the cells. If there are a lot of fl oating “dead” cells, change the medium. You can revert back to medium with 2.5% FBS + 2.5% CS at this point.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
MLO-Y4 cell line is useful to study effects of fluid flow and substrate stretching on activation of osteocyte-specific signaling pathways. Highlights: Derived from a transgenic mouse in which the immortalizing T-antigen was expressed under control of the osteocalcin promoterHigh expression of osteocalcinHigh expression of connexin 43 and the antigen E11Retain a dendritic morphology, similar to that observed in primary osteocyte Osteocytes are the most abundant bone cells in the body but also the most challenging to study because they are embedded in a mineralized matrix making them to difficult to isolate. This cell line MLO-Y4 makes it easier to study osteocyte function.Product Type: Cell Line Name: MLO-Y4 Cell Type: Osteocyte Organism: Mouse (female) Accession ID: CVCL_M098 Morphology: Dendritic morphology Biosafety Level: I Subculturing: Maintain stock cultures in complete culture medium at ~60-75% confluence on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:5 dilution (~1:7maximum) using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81 Growth Conditions: alpha-MEM, containing L-glutamine and deoxyribonucleosides, with penicillin/streptomycin at 100 U/ml and 2.5% FBS and 2.5% CS; both heat inactivated; Grown on dishes coated with [0.15 mg/ml] rat tail type 1 collagen (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81). Cryopreservation: 60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81) Comments: When first starting these cells, centrifuge at approx. 1,000 rpm, for 5–10 min. Aspirate media, gently resuspend and plate the cell pellet in media containing 5% FBS and 5% CS. This higher amount of serum, 10% total, is useful to give the cells an extra boost. The next day, check the viability of the cells. If there are a lot of fl oating “dead” cells, change the medium. You can revert back to medium with 2.5% FBS + 2.5% CS at this point.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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