Cloning vector pSIREN_U6-shRNA_FF6-CMV-iRFP质粒载体-BioVector NTCC保藏中心

Cloning vector pSIREN_U6-shRNA_FF6-CMV-iRFP质粒载体-BioVector NTCC保藏中心

Map图谱

Cloningvector pSIREN_U6-shRNA_FF6-CMV-iRFP, complete sequence

GenBank: KM486850.1

LOCUS      KM486850                6896bp    DNA     circular SYN 16-DEC-2014

DEFINITION Cloning vector pSIREN_U6-shRNA_FF6-CMV-iRFP, complete sequence.

ACCESSION  KM486850

VERSION    KM486850.1  GI:731516999

KEYWORDS   .

SOURCE     Cloning vector pSIREN_U6-shRNA_FF6-CMV-iRFP

ORGANISM  Cloning vectorpSIREN_U6-shRNA_FF6-CMV-iRFP

           other sequences; artificial sequences;vectors.

REFERENCE  1  (bases 1 to 6896)

AUTHORS   Li,Y., Jiang,Y.,Chen,H., Liao,W., Li,Z., Weiss,R. and Xie,Z.

TITLE     Modular construction ofmammalian gene circuits using TALE

          transcriptional repressors

JOURNAL   Unpublished

REFERENCE  2  (bases 1 to 6896)

AUTHORS   Li,Y., Jiang,Y., Chen,H.and Liao,W.

TITLE     Direct Submission

JOURNAL   Submitted (03-SEP-2014)Bioinformatics Division/Center for

          Synthetic & Systems Biology, Tsinghua National Laboratory for

          Information Science and Technology, Tsinghua University, Beijing

          100084, China

COMMENT     ##Assembly-Data-START##

          Sequencing Technology :: Sanger dideoxy sequencing

          ##Assembly-Data-END##

FEATURES            Location/Qualifiers

    source          1..6896

                    /organism="Cloningvector pSIREN_U6-shRNA_FF6-CMV-iRFP"

                    /mol_type="otherDNA"

                    /db_xref="taxon:1579883"

misc_feature    229..477

                    /note="Homo sapiensU6 promoter"

misc_feature    484..536

                   /note="shRNA-FF6"

misc_feature    741..1329

                    /note="CMV"

CDS             1361..2311

                    /codon_start=1

                    /transl_table=11

                    /product="iRFP"

                    /protein_id="AIZ66003.1"

                   /db_xref="GI:731517000"

                   /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG

                   SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAG

                    FIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVR

                   KITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRI

                   IPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGE

                    RLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"

CDS             complement(2462..3121)

                    /codon_start=1

                    /transl_table=11

                    /product="ChlR"

                    /protein_id="AIZ66004.1"

                    /db_xref="GI:731517001"

                   /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF

                   LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF

                   SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV

                    ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG

                    A"

CDS             5238..6098

                    /codon_start=1

                    /transl_table=11

                    /product="AmpR"

                    /protein_id="AIZ66005.1"

                   /db_xref="GI:731517002"

                   /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY

                   IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE

                    YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL

                   DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL

                   LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA

                    EIGASLIKHW"

rep_origin      6253..6872

                    /note="pBR322origin"

ORIGIN    

       1 tgctggcctt ttgctcacat gttctttcct gcgttatcccctgattctgt ggataaccgt

     61 attaccgccttacgcgtgta aaacgacggc cagtagatct gtaatacgac tcactatagg

    121 gcgctagctgctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg

    181 gccgcataacttcgtatagc atacattata cgaagttatc agtcgacggg caggaagagg

    241 ctatttcccatgattccttc atatttgcat atacgataca aggctgttag agagataatt

    301 agaattaatttgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa

    361 taatttcttgggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt

    421 accgtaacttgaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga

    481 tccgccaaagagattcctca taaattcaga ctttatgagg aatctctttg gtttttgaat

    541 tctctagaccattcgtttgg cgcgcgggcc cagtaggtaa gtgaacatgg tcatagctgt

    601 ttcctaggagatcctggtca tgactagtgc ttggattctc accaataaaa aacgcccggc

    661 ggcaaccgagcgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca

    721 acaggagtccaagcgagctc tagttattaa tagtaatcaa ttacggggtc attagttcat

    781 agcccatatatggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg

    841 cccaacgacccccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata

    901 gggactttccattgacgtca atgggtggag tatttacggt aaactgccca cttggcagta

    961 catcaagtgtatcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc

   1021 gcctggcattatgcccagta catgacctta tgggactttc ctacttggca gtacatctac

   1081 gtattagtcatcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga

   1141 tagcggtttgactcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg

   1201 ttttggcaccaaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg

    1261 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctggtttagtgaac

   1321 cgtcagatccgctagcgcta ccggactcag atccaccggt atggctgaag gatccgtcgc

   1381 caggcagcctgacctcttga cctgcgacga tgagccgatc catatccccg gtgccatcca

   1441 accgcatggactgctgctcg ccctcgccgc cgacatgacg atcgttgccg gcagcgacaa

   1501 ccttcccgaactcaccggac tggcgatcgg cgccctgatc ggccgctctg cggccgatgt

   1561 cttcgactcggagacgcaca accgtctgac gatcgccttg gccgagcccg gggcggccgt

   1621 cggagcaccgatcactgtcg gcttcacgat gcgaaaggac gcaggcttca tcggctcctg

   1681 gcatcgccatgatcagctca tcttcctcga gctcgagcct ccccagcggg acgtcgccga

   1741 gccgcaggcgttcttccgcc gcaccaacag cgccatccgc cgcctgcagg ccgccgaaac

   1801 cttggaaagcgcctgcgccg ccgcggcgca agaggtgcgg aagattaccg gcttcgatcg

   1861 ggtgatgatctatcgcttcg cctccgactt cagcggcgaa gtgatcgcag aggatcggtg

   1921 cgccgaggtcgagtcaaaac taggcctgca ctatcctgcc tcaaccgtgc cggcgcaggc

   1981 ccgtcggctctataccatca acccggtacg gatcattccc gatatcaatt atcggccggt

   2041 gccggtcaccccagacctca atccggtcac cgggcggccg attgatctta gcttcgccat

   2101 cctgcgcagcgtctcgcccg tccatctgga attcatgcgc aacataggca tgcacggcac

   2161 gatgtcgatctcgattttgc gcggcgagcg actgtgggga ttgatcgttt gccatcaccg

   2221 aacgccgtactacgtcgatc tcgatggccg ccaagcctgc gagctagtcg cccaggttct

   2281 ggcctggcagatcggcgtga tggaagagtg acaattgttg ttgttaactt gtttattgca

   2341 gcttataatggttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt

   2401 tcactgcattctagttgtgg tttgtccaaa ctcatcaatg tatcttgagc tcgatatcaa

   2461 attacgccccgccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga

   2521 catggaagccatcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt

   2581 cgccttgcgtataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg

   2641 ccacgtttaaatcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat

   2701 tctcaataaaccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg

   2761 aatatatgtgtagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg

   2821 tttcagtttgctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct

   2881 caccgtctttcattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt

   2941 gaataaaggccggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa

   3001 tatccagctgaacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat

   3061 gttctttacgatgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca

   3121 ttttagcttccttagctcct gaaagatcca taacttcgta tagcatacat tatacgaagt

   3181 tatgcggccgcgacgtccac atatacctgc cgttcactat tatttagtga aatgagatat

   3241 tatgatattttctgaattgt gattaaaaag gcaactttat gcccatgcaa cagaaactat

   3301 aaaaaatacagagaatgaaa agaaacagat agatttttta gttctttagg cccgtagtct

   3361 gcaaatccttttatgatttt ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa

   3421 acgagagtctaatagaatga ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc

   3481 aggcaagacctaaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc ccttacatat

   3541 tttaggtctttttttattgt gcgtaactaa cttgccatct tcaaacagga gggctggaag

   3601 aagcagaccgctaacacagt acataaaaaa ggagacatga acgatgaaca tcaaaaagtt

   3661 tgcaaaacaagcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca

   3721 agcgtttgcgaaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat

   3781 tacacgccatgatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc

   3841 tgagttcgattcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga

   3901 cagctggccattacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt

   3961 ctttgcattagccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca

   4021 aaaagtcggcgaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag

   4081 cgacaaattcgatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc

   4141 agccacatttacatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca

   4201 ttacggcaaacaaacactga caactgcaca agttaacgta tcagcatcag acagctcttt

   4261 gaacatcaacggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca

   4321 aaatgtacagcagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag

   4381 agatcctcactacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg

   4441 aactgaagatggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag

   4501 cacatcattcttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc

   4561 tgagttagcaaacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa

   4621 agtgatgaaaccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt

   4681 ctttaaaatgaacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat

   4741 tgacggcattacgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg

   4801 cccatacaagccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga

   4861 tgtaacctttacttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat

   4921 tacaagctatatgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgcctag

   4981 cttcctgctgaacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca

   5041 aggacaattaacagttaaca aataaaaacg caaaagaaaa tgccgatatc ctattggcat

    5101 tgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgtttatttttct

   5161 aaatacattcaaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat

   5221 attgaaaaaggaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg

   5281 cggcattttgccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg

   5341 aagatcagttgggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc

   5401 ttgagagttttcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat

   5461 gtggcgcggtattatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact

   5521 attctcagaatgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca

   5581 tgacagtaagagaattatgc agtgctgcca taaccatgag tgataacact gcggccaact

   5641 tacttctgacaacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg

   5701 atcatgtaactcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg

   5761 agcgtgacaccacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg

   5821 aactacttactctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg

   5881 caggaccacttctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag

   5941 ccggtgagcgtgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc

   6001 gtatcgtagttatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga

   6061 tcgctgagataggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat

   6121 atatactttagattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc

   6181 tttttgataatctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag

   6241 accccgtagaaaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct

   6301 gcttgcaaacaaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac

   6361 caactctttttccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc

   6421 tagtgtagccgtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg

   6481 ctctgctaatcctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt

   6541 tggactcaagacgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt

   6601 gcacacagcccagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc

   6661 tatgagaaagcgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca

   6721 gggtcggaacaggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata

   6781 gtcctgtcgggtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg

   6841 ggcggagcctatggaaaaac gccagcaacg cggccttttt acggttcctg gccttt

//