IMDC-MutuDC1940(Immortalized Mouse Dendritic Cells) cell line永生化小鼠树突状细胞-BioVector NTCC典型培养物保藏中心

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an immortalized splenic CD8α+subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice.The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout, TLR9 knockout and Ifnar1 knockout MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α+subset.

Mus musculus

Spleen

Adherent

Small Aggregates

Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene

CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Dry Ice.

Lenti-hTERT Immortalization Kit
Lenti-SV40T Immortalization Kit

For Research Use Only

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems;
2) proteome profile and surface markers assessed by RT-PCR; RT-PCR;
3) IL-12 cytokine secretion analyzed using ELISA;
4) Response to PAMP stimulation evaluated by functional assays.

II