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C3H10T1/2 cell line细胞株
C3H/10T1/2, Clone 8BioVector-CCL-226Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodMorphologyfibroblastTissueEmbryoDiseaseSarcomaApplications3D cell cultureProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenSpecific applicationsThis line is a suitable transfection host.Growth propertiesAdherentDerivationC3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.AgeembryoStrainC3HClinical dataThe depositor recommends that the line be used between the 5th and 15th passages only.KaryotypeMouse karyotype with a modal number of 80 chromosomes.TumorigenicNo;No, in immunosuppressed miceRefRefYes, in semisolid mediumRefRefCommentsThe cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.The cells are contact sensitive.There is no detectable background spontaneous transformation.They are highly susceptible to transformation by chemical agents.Tested and found negative for ectromelia virus (mousepox).Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics.The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.The depositor recommends that the line be used between the 5th and 15th passages only.Unpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Basal medium supplemented with heat-inactivated fetal bovine serum to a final concentration of 10% and 2mM L-glutamine.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureNever allow the culture to become completely confluent. Subcultivation of cultures must be performed before they reach confluence. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: Seed new flasks at 2000 viable cells/cm2.Medium Renewal: Once between subcultures if necessaryReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
C3H/10T1/2, Clone 8BioVector-CCL-226Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodMorphologyfibroblastTissueEmbryoDiseaseSarcomaApplications3D cell cultureProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenSpecific applicationsThis line is a suitable transfection host.Growth propertiesAdherentDerivationC3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.AgeembryoStrainC3HClinical dataThe depositor recommends that the line be used between the 5th and 15th passages only.KaryotypeMouse karyotype with a modal number of 80 chromosomes.TumorigenicNo;No, in immunosuppressed miceRefRefYes, in semisolid mediumRefRefCommentsThe cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.The cells are contact sensitive.There is no detectable background spontaneous transformation.They are highly susceptible to transformation by chemical agents.Tested and found negative for ectromelia virus (mousepox).Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics.The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.The depositor recommends that the line be used between the 5th and 15th passages only.Unpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Basal medium supplemented with heat-inactivated fetal bovine serum to a final concentration of 10% and 2mM L-glutamine.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureNever allow the culture to become completely confluent. Subcultivation of cultures must be performed before they reach confluence. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: Seed new flasks at 2000 viable cells/cm2.Medium Renewal: Once between subcultures if necessaryReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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