HUVEC/TERT2 cell line人脐带血管内皮永生化细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：HUVEC/TERT2
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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HUVEC/TERT2 cell line人脐带血管内皮永生化细胞株
Product categoryHuman cellsProduct typehTERT-immortalized cellOrganismHomo sapiens, humanCell typeendothelial cellMorphologyCobblestone appearance with large dark nucleiTissueUmbilical cord; Vascular endotheliumDiseaseNormalApplications3D cell cultureDrug developmentHigh-throughput screeningProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenSpecific applicationsImmune response, wound healing, cellular response to viral or bacterial infection, oxidative stress, angiogenesis, arteriolosclerosis, drug screening, tubule formationCharacteristicsCells per vialApproximately ≥ 1.2 x 106Volume1.0 mLGrowth propertiesAdherentDerivationUmbilical vascular endothelium transduced with hTERTAgeneonateEthnicityWhiteGenderFemaleImmortalization methodhTERT expressionKaryotype46,XX,add(14)(p10)Antigen expressionCD31 >75% positiveExpression markersLDL uptake >75% positiveUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Vascular Cell Basal Medium , supplemented with Vascular Endothelial Cell Growth Kit-VEGF . Alternatively, the Vascular Cell Basal Medium can be supplemented with Endothelial Cell Growth Kit-BBE .Handling procedureTo ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt.If storage of the frozen culture is necessary upon arrival, store the vial in liquid nitrogen vapor phase and NOTat –70°C. Storage at –70°C will result in loss of viability.Prepare a 25 cm2 or a 75 cm2 culture flask containing 8.0 x 103 to 10.0 x 103 viable cells/cm2 in the recommended complete culture medium. Prior to the addition of the vial contents, the vessel containing the growth medium should be placed in the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6) and to avoid excessive alkalinity of the medium during recovery of the cells.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL of complete culture medium and centrifuge the cell suspension at approximately 125 x g for 5 to 7 minutes.Discard the supernatant and resuspend the cells in fresh growth medium (see the batch-specific information for the recommended dilution ratio). Add this suspension to the prepared culture vessel.Incubate the culture at 37°C in a suitable incubator.Subculturing procedureEvery 3-4 days at 8000 cells/cm2 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.Remove and discard spent medium.Briefly rinse with 1X PBS, 1 mL/25 cm2 and discard rinse solution.Add trypsin for primary cells, 1 mL/25 cm2. Place at 37oC for 3-4 minutes, until 90% of the cells have detached. Rapflask gently to ensure cells are detached. Add 2% FBS in D-PBS, 1 mL/25 cm2 to neutralize trypsin.Centrifuge cells at 150 x g for 5 min at room temperature.Remove supernatant. Resuspend pellet in 5.0 to 8.0 mL Complete Growth Medium.Count cells, and seed 8.0 x 103 to 10.0 x 103 viable cells/cm2 to new culture vessels.Medium Renewal: Every 2-3 days.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Product categoryHuman cellsProduct typehTERT-immortalized cellOrganismHomo sapiens, humanCell typeendothelial cellMorphologyCobblestone appearance with large dark nucleiTissueUmbilical cord; Vascular endotheliumDiseaseNormalApplications3D cell cultureDrug developmentHigh-throughput screeningProduct formatFrozenStorage conditionsVapor phase of liquid nitrogenSpecific applicationsImmune response, wound healing, cellular response to viral or bacterial infection, oxidative stress, angiogenesis, arteriolosclerosis, drug screening, tubule formationCharacteristicsCells per vialApproximately ≥ 1.2 x 106Volume1.0 mLGrowth propertiesAdherentDerivationUmbilical vascular endothelium transduced with hTERTAgeneonateEthnicityWhiteGenderFemaleImmortalization methodhTERT expressionKaryotype46,XX,add(14)(p10)Antigen expressionCD31 >75% positiveExpression markersLDL uptake >75% positiveUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Vascular Cell Basal Medium , supplemented with Vascular Endothelial Cell Growth Kit-VEGF . Alternatively, the Vascular Cell Basal Medium can be supplemented with Endothelial Cell Growth Kit-BBE .Handling procedureTo ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt.If storage of the frozen culture is necessary upon arrival, store the vial in liquid nitrogen vapor phase and NOTat –70°C. Storage at –70°C will result in loss of viability.Prepare a 25 cm2 or a 75 cm2 culture flask containing 8.0 x 103 to 10.0 x 103 viable cells/cm2 in the recommended complete culture medium. Prior to the addition of the vial contents, the vessel containing the growth medium should be placed in the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6) and to avoid excessive alkalinity of the medium during recovery of the cells.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL of complete culture medium and centrifuge the cell suspension at approximately 125 x g for 5 to 7 minutes.Discard the supernatant and resuspend the cells in fresh growth medium (see the batch-specific information for the recommended dilution ratio). Add this suspension to the prepared culture vessel.Incubate the culture at 37°C in a suitable incubator.Subculturing procedureEvery 3-4 days at 8000 cells/cm2 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.Remove and discard spent medium.Briefly rinse with 1X PBS, 1 mL/25 cm2 and discard rinse solution.Add trypsin for primary cells, 1 mL/25 cm2. Place at 37oC for 3-4 minutes, until 90% of the cells have detached. Rapflask gently to ensure cells are detached. Add 2% FBS in D-PBS, 1 mL/25 cm2 to neutralize trypsin.Centrifuge cells at 150 x g for 5 min at room temperature.Remove supernatant. Resuspend pellet in 5.0 to 8.0 mL Complete Growth Medium.Count cells, and seed 8.0 x 103 to 10.0 x 103 viable cells/cm2 to new culture vessels.Medium Renewal: Every 2-3 days.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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