Human coronavirus OC43 病毒毒株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Human coronavirus OC43 病毒毒株

Product Sheet

Human coronavirus OC43, betacoronavirus 1

Cat No.: BioVector936218

Human coronavirus strain OC43 is propagated in HCT-8 [HRT-18] cells. This strain was isolated from a man with a cold-like illness. It has applications in respiratory disease research and infectious disease research.
Product category
Viruses
Product type
Animal virus
Classification
Coronaviridae, Betacoronavirus
Strain designation
OC43
Common name
Human coronavirus OC43
Type strain
No
Isolation source
Man with cold-like illness. Treated to remove mycoplasma at BioVector.
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder

Specific applications
Respiratory research

Characteristics
Comments

OC43 is a TC-adapted item derived from virus passaged in mice. Cleaned of mycoplasma with MRA. No antigenic cross reaction with Human coronavirus 229E.

Next-generation sequencing (NGS) at BioVector on the HCT-8 [HRT-18] cell line used as the host has shown the presence of a putative fragment of Gammaherpesvirus with a ≥98% homology to Human Heparanase 2 (NCBI number NG_023416.1).
Mycoplasma contamination
Not detected

Handling information
Host
HCT-8 [HRT-18] (BioVector CCL-244)
Effects
CPE; cell vacuolization; cell sloughing
Complete medium
RPMI-1640 + 2% HS (BioVector 30-2040)
Temperature
33°C
Incubation
4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Handling notes
OC43 is a TC-adapted item derived from virus passaged in mice (BioVector VR-759). Cleaned of mycoplasma with MRA. No antigenic cross reaction with Human coronavirus 229E.
Key abbreviations
RPMI, Roswell Park Memorial Institute
TC, Tissue culture
CPE, Cytopathic effect
HCT-8, adult human colon epithelial cells
HS, Horse serum
MOI, Multiplicity of infection
MRA, Mycoplasma removal agent
PBS, Phosphate-buffered saline
Quality control specifications
Volume
1.0 mL
Verification method
Whole-genome Sequencing

Type of isolate
Human
Patient gender
Male
Cross references
GenBank NC_005147.1 Human coronavirus OC43, complete genome

Images:

Level 2






Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net