- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
H28 cell line人恶性间皮瘤细胞株
H28 Cell LineCat No.: BioVector-A5820Product categoryHuman cellsOrganismHomo sapiens, humanTissueLungDiseaseMesothelioma; Stage 4Applications3D cell cultureCharacteristicsGrowth propertiesAdherentDerivationThe line was established in September 1976.Age48 yearsEthnicityWhiteGenderMaleClinical dataThe patient was a smoker.Karyotypet(3p;14q)MetastaticPleural effusionCommentsHandling informationUnpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to an appropriate size vessel. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).4.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.5.If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.Subculturing procedureVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.1.Remove and discard culture medium.2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.5.Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOQuality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: X,YCSF1PO: 10,11D13S317: 11,13D16S539: 12,13D5S818: 11D7S820: 10,12THO1: 7,9TPOX: 11vWA: 14,15HistoryDeposited asHomo sapiensDepositorsAF Gazdar, JD MinnaYear of origin1976Level 1
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
H28 Cell LineCat No.: BioVector-A5820Product categoryHuman cellsOrganismHomo sapiens, humanTissueLungDiseaseMesothelioma; Stage 4Applications3D cell cultureCharacteristicsGrowth propertiesAdherentDerivationThe line was established in September 1976.Age48 yearsEthnicityWhiteGenderMaleClinical dataThe patient was a smoker.Karyotypet(3p;14q)MetastaticPleural effusionCommentsHandling informationUnpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.Temperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to an appropriate size vessel. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).4.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.5.If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.Subculturing procedureVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.1.Remove and discard culture medium.2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.5.Add appropriate aliquots of the cell suspension to new culture vessels.6.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOQuality control specificationsMycoplasma contaminationNot detectedSTR profilingAmelogenin: X,YCSF1PO: 10,11D13S317: 11,13D16S539: 12,13D5S818: 11D7S820: 10,12THO1: 7,9TPOX: 11vWA: 14,15HistoryDeposited asHomo sapiensDepositorsAF Gazdar, JD MinnaYear of origin1976Level 1
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 H28 cell line人恶性间皮瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
