TT2609-C02 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

TT2609-C02 cell line细胞株

Cell line:
TT2609-C02
NTCC no.:
ACC 510
Species:
human (Homo sapiens)
Cell type:
follicular thyroid carcinoma
Origin:
established in 1996 from the tumor tissue of a 57-year-old man with local recurrence of a previously iodine-irradiated follicular thyroid cancer (well-differentiated with dispersed poorly differentiated areas). Cells express the transcriptionally inactive delta p53 isoform. A sister cell line with a truncated delta p53 -/- is also available (ACC 859). Useful for studies with p53 family members p63 and p73
Reference(s):
14552
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
small fibroblastoid cells growing adherent but irregularly in monolayers
Medium:
80-85% RPMI 1640 + 15-20% h.i. FBS + 1 mM sodium pyruvate + insulin-transferrin-selenite medium supplement
Subculture:
split confluent culture 1:3 to 1:4 every week using trypsin/EDTA (for 10 min); seed out at ca. 1 x 106 cells/25 cm2; maintain at ca. 8 x 106 cells/175 cm2; maximal density at about 14 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
cell harvest of about 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 4% polyploidy - 69-75<3n>XXYY, -2, +8, +8, +9, +10, +14, -15, -16, +17, -22, +1-2mar, der(1)t(1;2)(p32;q11-13), add(4)(p15.2), ?inv(8)(q12q21.2)x1-2, add(9)(p24)x2, i(15q), del(17)(p11)x1-2, add(22)(p11)x2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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