P2Y2 (human) expressing cell line，Purinergic P2Y2 (human) cell line稳定表达细胞株GPCR BioVector NTCC质粒载体菌种细胞基因保藏中心

P2Y2 (human) expressing cell line，Purinergic P2Y2 (human) cell line稳定表达细胞株GPCR

P2Y2 expressing GPCR frozen cells are designed by BioVector NTCC Inc. for simple, rapid calcium assays with no requirement for intensive cell culturing. BioVector has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The P2Y GPCRs serve as receptors for extracellular nucleotides (Abbracchio et al., 2006). P2Y2 (also known as P2U) is activated by UTP and ATP, and couples to Gq to increase intracellular calcium (Parr et al., 1994). The P2Y2 receptor knockout mouse displays defective nucleotide-stimulated Cl- secretion, and a P2Y2 selective agonist increases tracheal epithelial chloride and water secretion (Cressman et al., 1999; Yerxa et al., 2002). As a result, P2Y2 is a potential target for treatment of cystic fibrosis. In addition, the P2Y2 KO mouse displays salt-resistant hypertension (Rieg et al., 2007). BioVector’s cloned human P2Y2-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y2 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y2.

Application Human P2Y2 GPCR cells for Calcium Flux FLIPR Assays.
Key Applications Calcium Flux Assays

Biological Information
Entrez Gene Number
NM_002564.2
GPCR Class A
Host Cells 1321N1
Protein Target P2Y2
Target Sub-Family P2Y / Purinergic (metabotropic)
Cell Line Type
GPCR Cell Lines

BioVector’s P2Y2 Cells Growth Medium

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net