B.subtilis RIK1285 strain枯草芽孢杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：B.subtilis RIK1285
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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B.subtilis RIK1285 strain枯草芽孢杆菌菌株
B. subtilis Secretory Protein Expression SystemBacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, may be required to achieve optimal yield. To address this concern, the B. Subtilis Secretory Protein Expression System from BioVector NTCC Inc. allows rapid development of a B. subtilis clone library, with each clone bearing a pBE-S construct in which the ORF of your protein of interest is fused with 173 different sequences for unique signal peptides. By performing a downstream assay to identify and select the clone that secretes the highest amount of functional protein, you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.OverviewIncludes pBE-S DNA, an E. coli/B. subtilis shuttle vector containing the B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), multiple cloning site (MCS) and 3' (C-terminal) his-tag sequenceSupplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target geneFully compatible with In-Fusion Cloning kits and systems, allowing rapid and easy construct generationIncludes B. subtilis strain RIK1285More InformationApplicationsOptimize secretion by identifying the signal peptide which provides the highest level of expression of secreted proteinThe clone with the highest level of expression is then ready to use for scaled-up protein expression using B. subtilisComponentsSP DNA Mixture (0.032 pmol/µl) 45 µlpBE-S DNA (0.5 µg/µl) 20 µgB. subtilis RIK1285 (Glycerol Stock) 100 µl x 2NotesThe B. subtilis Secretory Protein Expression System provides sufficient reagents for 10 library construction reactions.Genotype of B. subtilis RIK1285: Marburg 168 derivative: trpC2, ys1, aprEdelta3, nprR2, nprE18
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
B. subtilis Secretory Protein Expression SystemBacillus subtilis has become an increasingly popular host for recombinant protein expression. With its ability to secrete protein directly into culture media, amenability to medium- and large-scale fermentation, lack of codon bias, and designation by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS), it's no wonder that the majority of industrially-produced enzymes are expressed in Bacillus species such as B. subtilis. Optimization of secretion, however, may be required to achieve optimal yield. To address this concern, the B. Subtilis Secretory Protein Expression System from BioVector NTCC Inc. allows rapid development of a B. subtilis clone library, with each clone bearing a pBE-S construct in which the ORF of your protein of interest is fused with 173 different sequences for unique signal peptides. By performing a downstream assay to identify and select the clone that secretes the highest amount of functional protein, you can quickly identify which signal peptide results in the most efficient expression of your desired secreted protein.OverviewIncludes pBE-S DNA, an E. coli/B. subtilis shuttle vector containing the B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), multiple cloning site (MCS) and 3' (C-terminal) his-tag sequenceSupplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target geneFully compatible with In-Fusion Cloning kits and systems, allowing rapid and easy construct generationIncludes B. subtilis strain RIK1285More InformationApplicationsOptimize secretion by identifying the signal peptide which provides the highest level of expression of secreted proteinThe clone with the highest level of expression is then ready to use for scaled-up protein expression using B. subtilisComponentsSP DNA Mixture (0.032 pmol/µl) 45 µlpBE-S DNA (0.5 µg/µl) 20 µgB. subtilis RIK1285 (Glycerol Stock) 100 µl x 2NotesThe B. subtilis Secretory Protein Expression System provides sufficient reagents for 10 library construction reactions.Genotype of B. subtilis RIK1285: Marburg 168 derivative: trpC2, ys1, aprEdelta3, nprR2, nprE18
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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