pSIP400 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSIP400 plasmid vector质粒载体

spp-based expression vector, 5.5 kb, EmR; SppKR expression driven by ermL read-through .
Vectors for inducible expression of genes in Lactobacillus sakei and Lactobacillus plantarum. The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and sakacin P and the genes encoding the cognate histidine protein kinase and response regulator that are necessary to activate this promoter upon induction by a peptide pheromone. The vectors are built up of cassettes that permit easy exchange of all parts through restriction enzyme digestion and ligation. Using β-glucuronidase as a reporter enzyme, variants of these vectors were compared with each other, and with a corresponding system based on genes involved in the production of nisin. Several of the new vectors permitted tightly controlled and efficient expression of β-glucuronidase in both L. sakei and L. plantarum.

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