Dubca Cell Line骆驼皮肤成纤维细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥59865
- 货 号：Dubca cell
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
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手机：18901268599
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Dubca Cell Line骆驼皮肤成纤维细胞株
Dubca Cell LineCat No.: BioVector-A2276Product categoryAnimal cellsOrganismCamelus dromedarius, camelMorphologyfibroblastTissueSkinDiseaseAutopsyApplications3D cell cultureVaccine developmentSpecific applicationsSV40 large T-antigen is detected by Western blotting.The cell line is susceptible to infection with viruses of several different families.The cells may be used for virus diagnosis and vaccine production.Growth propertiesMonolayerDerivationThe cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.Age2 months gestationGenderFemaleImmortalization methodSV40 transformedCommentsThe cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.SV40 large T-antigen is detected by Western blotting.The cell line is susceptible to infection with viruses of several different families.Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen).The cells may be used for virus diagnosis and vaccine production.The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Unpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Handling procedureHANDLING PROCEDURE FOR FROZEN CELLS- Initiate culture as soon as possible upon receipt.- Thaw by rapid agitation in 37°C water bath. Thawing should be rapid (within40-60 seconds). As soon as the ice is melted, remove the ampule from the waterbath. All of the operations from this point on should be carried out understrict aseptic conditions.- Transfer the cell suspension and dilute it with the recommended culturemedium in a culture flask (see specific batch information above for dilutionratio); incubate at 37°C with 5% CO2 in air atmosphere. Since it is importantto avoid excessive alkalinity of the medium during recovery of the cells, it issuggested that the culture medium be placed into the culture flask, tube, etc.and the pH be adjusted, as necessary, prior to the addition of the vialcontents. Note that the bicarbonate content of the culture medium willdetermine whether an atmosphere containing CO2 will be required.- It is not necessary to remove the freezing additive. However, if desired,the culture medium may be changed to remove the protective freezing additive(dimethylsulfoxide) 24 hours after thawing. If it is desired that the freezingadditive be removed immediately, or that a more concentrated cell suspension beobtained, centrifuge the above diluted suspension at approximately 125 x g for10 minutes, discard the fluid and resuspend the cells with growth medium at thedilution ratio given in the specific batch information above.FLUID RENEWALEvery 2-3 days.SUBCULTURE PROCEDURERemove medium, rinse with trypsin (0.25%) - EDTA (0.03%) solution. Add 1-2 mladditional trypsin solution and allow flasks to remain at room temperature (orincubate at 37°C) until cells detach. Add fresh culture medium, aspirate anddispense into new culture flasks.Subcultivation ratio: 1:4 to 1:6 is recommended.Subculturing procedureSubcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: Every 2 to 3 daysRemove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOLevel 2
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Dubca Cell LineCat No.: BioVector-A2276Product categoryAnimal cellsOrganismCamelus dromedarius, camelMorphologyfibroblastTissueSkinDiseaseAutopsyApplications3D cell cultureVaccine developmentSpecific applicationsSV40 large T-antigen is detected by Western blotting.The cell line is susceptible to infection with viruses of several different families.The cells may be used for virus diagnosis and vaccine production.Growth propertiesMonolayerDerivationThe cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.Age2 months gestationGenderFemaleImmortalization methodSV40 transformedCommentsThe cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.SV40 large T-antigen is detected by Western blotting.The cell line is susceptible to infection with viruses of several different families.Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen).The cells may be used for virus diagnosis and vaccine production.The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Unpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Handling procedureHANDLING PROCEDURE FOR FROZEN CELLS- Initiate culture as soon as possible upon receipt.- Thaw by rapid agitation in 37°C water bath. Thawing should be rapid (within40-60 seconds). As soon as the ice is melted, remove the ampule from the waterbath. All of the operations from this point on should be carried out understrict aseptic conditions.- Transfer the cell suspension and dilute it with the recommended culturemedium in a culture flask (see specific batch information above for dilutionratio); incubate at 37°C with 5% CO2 in air atmosphere. Since it is importantto avoid excessive alkalinity of the medium during recovery of the cells, it issuggested that the culture medium be placed into the culture flask, tube, etc.and the pH be adjusted, as necessary, prior to the addition of the vialcontents. Note that the bicarbonate content of the culture medium willdetermine whether an atmosphere containing CO2 will be required.- It is not necessary to remove the freezing additive. However, if desired,the culture medium may be changed to remove the protective freezing additive(dimethylsulfoxide) 24 hours after thawing. If it is desired that the freezingadditive be removed immediately, or that a more concentrated cell suspension beobtained, centrifuge the above diluted suspension at approximately 125 x g for10 minutes, discard the fluid and resuspend the cells with growth medium at thedilution ratio given in the specific batch information above.FLUID RENEWALEvery 2-3 days.SUBCULTURE PROCEDURERemove medium, rinse with trypsin (0.25%) - EDTA (0.03%) solution. Add 1-2 mladditional trypsin solution and allow flasks to remain at room temperature (orincubate at 37°C) until cells detach. Add fresh culture medium, aspirate anddispense into new culture flasks.Subcultivation ratio: 1:4 to 1:6 is recommended.Subculturing procedureSubcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: Every 2 to 3 daysRemove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.Reagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSOLevel 2
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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