mm.1r-luc cell line荧光稳定表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989865
- 货 号：mm.1r-luc
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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mm.1r-luc cell line荧光稳定表达细胞株
Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphoblastMorphologylymphoblastTissuePeripheral bloodDiseaseMultiple MyelomaApplications3D cell cultureImmune system disorder researchImmunologySpecific applicationsMM.1R is resistant to dexamethasone.Growth propertiesMixed: suspension with some loosely adherent cellsDerivationThe parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy.Age42 yearsEthnicityBlackGenderFemaleAntigen expressionCD25-, CD38+, CD52+, CD59+RefGenes expressedlambda-light chain immunoglobulinExpression markersGlucocorticoid receptor, not expressedCommentsMM.1R is resistant to dexamethasone. The closely related cell line, MM.1S was also isolated from MM.1, but is sensitive to dexamethasone.Unpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a new culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureThis is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.Note: Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended.Medium renewal: Every 2 to 3 days.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Product categoryHuman cellsOrganismHomo sapiens, humanCell typeB lymphoblastMorphologylymphoblastTissuePeripheral bloodDiseaseMultiple MyelomaApplications3D cell cultureImmune system disorder researchImmunologySpecific applicationsMM.1R is resistant to dexamethasone.Growth propertiesMixed: suspension with some loosely adherent cellsDerivationThe parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy.Age42 yearsEthnicityBlackGenderFemaleAntigen expressionCD25-, CD38+, CD52+, CD59+RefGenes expressedlambda-light chain immunoglobulinExpression markersGlucocorticoid receptor, not expressedCommentsMM.1R is resistant to dexamethasone. The closely related cell line, MM.1S was also isolated from MM.1, but is sensitive to dexamethasone.Unpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a new culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureThis is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.Note: Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended.Medium renewal: Every 2 to 3 days.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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