Eubacterium dolichum strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Eubacterium dolichum strain菌株

Eubacterium dolichum Moore et al.
Cat# BioVector-29143

Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI C9-20 [DSM 3991]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Product format
Freeze-dried

Comments
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.

Medium 593: Chopped meat medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.

3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.

4. Incubate tubes and one plate under anaerobic conditions at 37°C. Incubate one blood plate aerobically at 37°C.

5. Within 48 hours, growth should be evident by light turbidity in the broth and pinpoint colonies on anaerobic agar surfaces. No growth should occur on the blood agar plate incubated aerobically.

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

· Use of an anaerobic gas chamber, or

· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

· Loose screw caps on test tubes in anaerobic chamber,

· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or

· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

Handling notes
Colonies are very small. Cells are medium to long, thin rods occurring singly, in end to end pairs and V-formations.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net