Bacteroides stercoris VPI B5-21 strain粪便拟杆菌菌株BioVector43183 BioVector NTCC质粒载体菌种细胞基因保藏中心

Bacteroides stercoris VPI B5-21 strain粪便拟杆菌菌株BioVector43183

Bacteroides stercoris Johnson et al.
Cat# BioVector43183

Type strain.
Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI B5-21 [CIP 104203T, JCM 9496, subsp. a]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Geographical isolation
United States; Texas
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Comments
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1490 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1490 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24-48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas
chamber or placement of test tubes under a gassing cannula system connected to
anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
Anaerobe Systems Brucella Blood plate can be used to analyze colony morphology and purity.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

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