Clostridioides difficile (Prevot) strain菌株ATCC 9689 BioVector NTCC质粒载体菌种细胞基因保藏中心

Clostridioides difficile (Prevot) strain菌株ATCC 9689

Clostridioides difficile strain 90556-M6S [L.S. McClung 1780] is a whole-genome sequenced bacterial type strain with applications in enteric and infectious disease research. This strain is confirmed to have the tcdA and tcdB genes.
Product category
Bacteria
Product type
Anaerobe
Strain designation
90556-M6S [L.S. McClung 1780]
Type strain
Yes
Genome sequenced strain
Yes
Applications
Bioinformatics
Enteric disease research
Infectious disease research
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Specific applications
Produces cytotoxin
Enteric Research

Characteristics
Genotype
Toxinotype: 0
Toxigenic
Yes
Toxin genes
cdtB (Binary toxin) negative
tcdA (Toxin A) positive
tcdB (Toxin B) positive
Comments
Presence of tcdA and tcdB genes confirmed by PCR. Ref
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #260 plate.
Incubate in an anaerobic atmosphere at 37°C for 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
Anaerobe Systems Brucella Blood Plate (AS-111 or AS-141) can be used to analyze colony morphology and purity.
The presence of tcdA and tcdB genes is confirmed by PCR. The binary toxin gene cdtB is not amplified by PCR.
Purified genomic DNA of this strain is available

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