Clostridium sporogenes strain生孢梭菌标准菌株ATCC11437 BioVector NTCC质粒载体菌种细胞基因保藏中心

Clostridium sporogenes strain生孢梭菌标准菌株ATCC11437

This organism is used for sterility testing, media testing, and quality control. Heat-resistant.
Product category
Bacteria
Product type
Anaerobe
Strain designation
L.S. McClung 2006
Type strain
No
Isolation source
Cotton plant
Applications
Media testing
Quality control
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Specific applications
Media testing
Quality control
Sterility testing
Pharmaceutical and Personal Care

Comments
Heat-resistant

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a brucella blood plate and/or blood agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 to 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Handling notes
Anaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology and purity. Anaerobe Systems Reinforced Clostridial Broth (AS-606) is recommended
for better growth.

Always use freshly prepared prereduced media or prereduced media that has been previously prepared but stored under anaerobic conditions.

Purified genomic DNA of this strain is available

Supplier来源:BioVector NTCC Inc.
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