Clostridium sporogenes strain生孢梭菌标准菌株ATCC 19404 BioVector NTCC质粒载体菌种细胞基因保藏中心

Clostridium sporogenes strain生孢梭菌标准菌株ATCC 19404

Bacterial anaerobe isolated from a human with an acute case of gas gangrene. This strain has applications as a quality control organism in media and sterility testing.
Product category
Bacteria
Product type
Anaerobe
Preceptrol culture
Strain designation
SR 5
Type strain
No
Isolation source
Human with an acute case of gas gangrene
Applications
Bioinformatics
Media testing
Quality control
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Specific applications
Media testing
Quality control strain
Sterility testing
Pharmaceutical and Personal Care

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions.
Under anaerobic conditions, withdraw 0.5 mL of #2107 broth from a single test tube (5 to 6 mL), with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a pre-reduced Brucella blood plate (Anaerobe Systems, AS-111 or AS-141) and a #260 slant. An aerobic blood plate may also be streaked to check for purity.
Incubate the tubes and plate under anaerobic conditions at 37°C for 24-48 hours. Incubate the purity plate aerobically at 37°C.




ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

Use of an anaerobic gas chamber, or
Placement of test tubes under a gassing cannula system connected to anaerobic gas.




Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in anaerobic chamber,
Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
ATCC recommends Anaerobe Systems PRAS Brucella Blood Plates (AS-111 or AS-141).
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

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