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A20 [A-20] Cell Line细胞株
A20 [A-20] Cell LineCat No.: BioVector-931722Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodCell typeB lymphocyteMorphologylymphoblastDiseaseSarcoma; Reticulum CellApplications3D cell cultureImmunologySpecific applicationsThis cell line is a suitable transfection host.CharacteristicsGrowth propertiesSuspensionDerivationThe A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.StrainBALB/cAnNKaryotypediploid; modal number = 37; range = 33 to 38TumorigenicYes;YesAntigen expressionI-A+ Genes expressedimmunoglobulin (surface, sIg+) I-A+Expression markersFcIsotypeIgGCommentsThe cells express little surface immunoglobulin when grown in Click's medium; however, they express large amounts when grown in RPMI 1640 medium.The cells can present both alloantigens and protein antigens.Tested and found negative for ectromelia virus (mousepox).DMEM-H +10%FBS+ 100 U/ml penicillin G sodium (option); 100 µg/ml streptomycin sulfate (option);Complete growth medium, 95%; DMSO, 5%Handling informationUnpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line isRPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium:·fetal bovine serum to a final concentration of 10%·0.05 mM 2-mercaptoethanolTemperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to a centrifuge tube containing 9.0 ml complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.4.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into new culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).5.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.Population doubling time: Approximately 18 hrsMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
A20 [A-20] Cell LineCat No.: BioVector-931722Product categoryAnimal cellsOrganismMus musculus, mouseClassificationEukaryota, Animalia, Metazoa, Chordata, Vertebrata, TetrapodCell typeB lymphocyteMorphologylymphoblastDiseaseSarcoma; Reticulum CellApplications3D cell cultureImmunologySpecific applicationsThis cell line is a suitable transfection host.CharacteristicsGrowth propertiesSuspensionDerivationThe A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.StrainBALB/cAnNKaryotypediploid; modal number = 37; range = 33 to 38TumorigenicYes;YesAntigen expressionI-A+ Genes expressedimmunoglobulin (surface, sIg+) I-A+Expression markersFcIsotypeIgGCommentsThe cells express little surface immunoglobulin when grown in Click's medium; however, they express large amounts when grown in RPMI 1640 medium.The cells can present both alloantigens and protein antigens.Tested and found negative for ectromelia virus (mousepox).DMEM-H +10%FBS+ 100 U/ml penicillin G sodium (option); 100 µg/ml streptomycin sulfate (option);Complete growth medium, 95%; DMSO, 5%Handling informationUnpacking and storage instructions1.Check all containers for leakage or breakage.2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line isRPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium:·fetal bovine serum to a final concentration of 10%·0.05 mM 2-mercaptoethanolTemperature37°CHandling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.3.Transfer the vial contents to a centrifuge tube containing 9.0 ml complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.4.Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into new culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).5.Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.Population doubling time: Approximately 18 hrsMedium Renewal: Every 2 to 3 daysReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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