Geobacter lovleyi strain菌株

Geobacter lovleyi
Cat# BioVector961151

Type strain. Genome sequenced strain.
Product category
Bacteria
Product type
Extremophile
Strain designation
SZ
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Su-Zi Creek
Geographical isolation
South Korea
Product format
Freeze-dried
Storage conditions
2°C to 8°C

BSL 1

Specific applications
Dechlorinates polychloroethylenes (PCE)
Reduces iron (ferric, FeIII) ferric iron

Characteristics
Extremophile type
Metallotolerant
Comments
Genome sequenced strain

Medium 2635: Desulfuromonas michiganenesis Medium
Temperature
26°C
Atmosphere
80% N2, 20% CO2
Handling procedure
Sterilize the top of the Balch tube by spraying with 70% ethanol and then flaming
If needed, exchange the gas in the test tube for 80% N2-20% CO2. Supplement the media with 200 µL per 100 mL PCE and 100 µL per 10 mL of fumarate.
When the Balch tube is ready to inoculate, open the vial according to enclosed instructions.
For inoculation, use an anaerobic 1.0 mL syringe tipped with 22-gauge needle. Withdraw 0.5 mL of #2635 broth and use this to rehydrate the entire freeze-dried pellet. Immediately place the rehydrated vial under a gentle stream of sterile oxygen-free gas.
Using the same syringe, transfer the rehydrated cell suspension to a tube of # 2635 broth. Plate 0.1 mL of the inoculated culture onto a non-selective medium and incubate aerobically at 26°C. Use 0.1 mL of the inoculated culture to inoculate a nonselective aerobic broth and an additional tube of #2635 broth. Incubate the broth tubes at 26°C.
Growth should be detected in the #2635 broth within 2 weeks. There should be no growth detected on the aerobic plate or in the aerobic broth.
Handling notes
After two weeks of incubation, growth can be viewed as motile rods in a wet mount. The broth will not become turbid. An “oil slick” may form at the surface of the broth due to the addition of PCE.



This organism is capable of degrading a common groundwater contaminant by completely dechlorinating tetrachloroethene (PCE). Growth on PCE yields very low cell numbers, but when supplemented with 10 mM fumarate, cell yields are much higher. Growth on media lacking PCE may decrease the cells’ ability to dechlorinate PCE.

Cross references
GenBank CP001089 Geobacter lovleyi SZ, complete genome.

洛夫利地杆菌是革兰氏阴性的金属还原性四氯乙烯脱氯的蛋白细菌。它作为一个有机体的生物修复潜力，并正在积极研究这样。


Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net