pET-41a,b,c plasmid vector原核表达载体GST标签质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pET-41a,b,c plasmid vector原核表达载体GST标签质粒

Description
The pET-41 series is designed for cloning and high-level expression of peptide sequences
fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the
sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the
circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase
is shown below. The f1 origin is oriented so that infection with helper phage will produce virions
containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded
sequencing should be performed using the T7 terminator primer.Vector encoded
sequence can be completely removed when cloning into the PshAI site (as shown below) and then
cleaving the GST fusion protein with Enterokinase.

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