pCITE-2a,pCITE-2b,pCITE-2c plasmid哺乳动物细胞体外翻译载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCITE-2a,pCITE-2b,pCITE-2c plasmid哺乳动物细胞体外翻译载体质粒

The pCITE-2a-c(+) vectors are designed for enhanced translation of cloned sequences in vitro using mammalian cell extracts, e.g. rabbit reticulocyte lysate. Unique sites are shown on the circle map below. The maps for pCITE-2b(+) (Cat. No. 69291-3) and pCITE-2c(+) are the same as pCITE-2a(+) (shown) with the following exceptions: pCITE-2b(+) is a 3793 bp plasmid; it lacks Nde I, EcoR V, BstX I and Xcm I sites. Subtract 7 bp from all sites beyond Msc I at 522. pCITE-2c(+) is a 3795 bp plasmid; it lacks Nde I, EcoR V, BstX I and Xcm I sites. Subtract 5 bp from all sites beyond Msc I at 522. The f1 origin in pCITE-2a-c(+) vectors is oriented so that infection with helper phage wil produce virions containing sinfgle stranded DNA that is complementary to the strand shown below. Therefore, single stranded sequencing should be performed using the CITE primer

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