hERα::Y190 strain酿酒酵母表达菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989865
- 货 号：hERα::Y190
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
hERα::Y190 strain酿酒酵母表达菌株
Estrogen receptors (ERs) are ligand-activated transcription factors that regulate gene expression and cell growth. Two ERs now have been identified: ERα and the more recently discovered ERβ . The physiological function of ERβ remains unclear, but evidence from vascular injury studies and from ERβ knockout mice suggests that ERβ may be involved in the regulation of cellular proliferation. There is a direct and specific interaction between ERβ and the cell cycle mitotic spindle assembly checkpoint protein, MAD2 (mitosis arrest-deficient 2). The ERβ -MAD2 interaction was identified by screening of a yeast two-hybrid system vascular endothelial cell library with ERβ and confirmed with glutathione S-transferase-fusion protein interaction studies. In contrast, ERα did not interact with MAD2 in either the two-hybrid system or in the protein-protein interaction experiments. Amino acids 173-208 in the hinge region of ERβ were sufficient to mediate the interaction with MAD2 in the two-hybrid system and in glutathione S-transferase-fusion protein studies.The full-length coding region of human ER alpha (hERα) was cloned in frame into the vector pBioVector-GBT9. The recombinated plasmid was transformed into Saccharomyces cerevisiae strain Y190 using the lithium acetate method.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Estrogen receptors (ERs) are ligand-activated transcription factors that regulate gene expression and cell growth. Two ERs now have been identified: ERα and the more recently discovered ERβ . The physiological function of ERβ remains unclear, but evidence from vascular injury studies and from ERβ knockout mice suggests that ERβ may be involved in the regulation of cellular proliferation. There is a direct and specific interaction between ERβ and the cell cycle mitotic spindle assembly checkpoint protein, MAD2 (mitosis arrest-deficient 2). The ERβ -MAD2 interaction was identified by screening of a yeast two-hybrid system vascular endothelial cell library with ERβ and confirmed with glutathione S-transferase-fusion protein interaction studies. In contrast, ERα did not interact with MAD2 in either the two-hybrid system or in the protein-protein interaction experiments. Amino acids 173-208 in the hinge region of ERβ were sufficient to mediate the interaction with MAD2 in the two-hybrid system and in glutathione S-transferase-fusion protein studies.The full-length coding region of human ER alpha (hERα) was cloned in frame into the vector pBioVector-GBT9. The recombinated plasmid was transformed into Saccharomyces cerevisiae strain Y190 using the lithium acetate method.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
- 公告/新闻
