Pimelobacter simplex strain菌株6946 BioVector NTCC质粒载体菌种细胞基因保藏中心

Pimelobacter simplex strain菌株6946

Pimelobacter simplex (Jensen) Suzuki and Komagata
BioVector-6946

Product category
Bacteria
Strain designation
NCTC 4215 [BUCSAV 385, CDA 835, FHP 534, NCIB 8929]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Rice soil
Product format
Freeze-dried

General
Specific applications
Degrades levulinic acid
Produces 3-keto-delta-1,4 steroids
Produces 5'-nucleotides
Produces 7-cyano steroids
Produces oxoalkylxanthines oxoalkylxanthine
Produces pregnadienes
Produces 5'-nucleotides by growth on hydrocarbons and subsequent degradation of intracellular RNA at alkaline pH
Reduction of 3-methoxy-8(14)-seco-1,3,5(10),9(11)-estratetraene-14, 17-dione
Reduction of 4-chloro-1-(4-fluorophenyl)butan-1-one
Dehydrogenation of Reichstein's compound S and hydrocortisone

Medium 3: Nutrient agar or nutrient broth
Temperature
26°C
Handling procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.

3. Aseptically transfer this aliquot back into the broth tube. Mix well.

4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.

5. Incubate the tubes and plate at 26oC for 48 hours.

Handling notes
Colonies on #3 agar are entire, glistening, circular, smooth, convex, and translucent.

Quality control specifications
Verification method
Whole-genome Sequencing

Type of isolate
Environmental
Cross references
GenBank M16174 P.simplex 5S rRNA.
GenBank M37693 Arthobacter simplex small subunit ribosomal RNA.


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