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Human Chondrocytes: Re-Differentiated: HC-RD cells人再分化型软骨细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99865
  • 货  号:Human Chondrocytes: Re-Differentiated: HC-RD
  • 产  地:北京
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BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

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地址:北京

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Human Chondrocytes: Re-Differentiated: HC-RD cells人再分化型软骨细胞

DESCRIPTION
Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension. HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen. RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.
Instructions to release HC-RD from Alginate Beads
Remove Chondrocyte Differentiation Medium
Wash the beads 2 times with 0.155 M NaCl
Add 3 volume of Depolymerization Solution per volume of packed alginate beads.
Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved
Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes
Wash the cells 2 times with 0.155 M NaCl
Media Salts & Chemicals used to Release HC-RD from alginate beads:
Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.
Sodium Alginate: Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes
Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.

Re-Differentiated Human Chondrocytes (HC-RD), encapsulated in an alginate bead suspension.
DETAILS
Tissue
Normal healthy human articular cartilage
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
N/A
Kit
N/A
Proliferating
Shipped in Differentiation Medium (flasks)
Doublings
N/A
Note
Cells shipped 10 days after encapsulation. Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation

Supplier来源:BioVector NTCC Inc.
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