Malassezia globosa strain球形马拉色菌菌株MYA-4612 / CBS 7966 BioVector NTCC质粒载体菌种细胞基因保藏中心

Malassezia globosa strain球形马拉色菌菌株MYA-4612 / CBS 7966

An ampoule containing viable cells (may include spores and mycelium) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Strain designation
CBS 7966 [ATCC 96807, GM 35]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Skin of patient with Pityriasis versicolor
Geographical isolation
England; London
Product format
Frozen

Characteristics
Comments
Lipophilic or lipid-dependent yeasts
Genome sequencing strain (The Procter & Gamble Company, USA; Genome Institute of Singapore, Singapore)
Opportunistic pathogen that can cause systematic infection in neonates and are associated with multiple human skin disorders

Medium 2693: modified Dixon (mDixon)
Temperature
30-35°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Lipophilic or lipid-dependent yeasts; Genome sequencing strain (The Procter & Gamble Company, USA); Opportunistic pathogen that can cause systematic infection in neonates and are associated with multiple human skin disorders.
If having trouble reviving this strain, try using liquid media with shaking; subculture at intervals of 2 weeks after recovering.

Type of isolate
Human
Cross references
GenBank GU291287 D1/D2 region of 28S rRNA gene
GenBank GU291271 ITS including 5.8S rRNA gene
GenBank AAYY00000000 Malassezia globosa CBS 7966, whole genome shotgun sequencing project

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