Brevendimonas diminuta FDA strain PCI 818 缺陷短波单胞菌菌株19146 BioVector NTCC质粒载体菌种细胞基因保藏中心

Brevendimonas diminuta FDA strain PCI 818 缺陷短波单胞菌菌株19146

Formerly Pseudomonas diminuta. Used for sterility assurance and membrane filter testing.
Product category
Bacteria
Strain designation
FDA strain PCI 818 [CCUG 24715, DSM 1635, LMG 10743]
Type strain
No
Isolation source
Contaminant in culture of Bacillus cereus
Applications
Bioinformatics
Product format
Freeze-dried

Specific applications
Pharmaceutical and Personal Care
Filtration sterilization control Ref
Membrane filter testing Ref Ref Ref Ref
Sterility assurance Ref Ref
Testing

Characteristics
Comments
Does not pass through a 0.2 micron membrane filter

Medium 3: Nutrient agar or nutrient broth
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Rehydrate the entire pellet with approximately 0.5 mL of #2495 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
Incubate at 30°C for 48-72 hours.
Handling notes
Phosphate buffer is for rehydration and transfer only, not for growth.
Two colony types may be found in this strain if it is passed through Nutrient Broth (BD 234000).
To prevent proliferation of the second colony type, rehydrate the vial as above and maintain the culture by transferring from agar to agar.
Purified genomic DNA of this strain is available

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