Faecalibacterium prausnitzii 27766 strain普拉梭菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Faecalibacterium prausnitzii 27766 strain普拉梭菌菌株

Faecalibacterium prausnitzii
27766

Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI C13-20-A [ATCC 29739]
Type strain
No
Genome sequenced strain
Yes
Isolation source
Feces

Medium 1703: Chopped meat carbohydrates with rumen fluid
Temperature
37°C
Atmosphere
97% CO2, 3% H2
Handling procedure
Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of #1703 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a #1703 plate and/or #1703 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 to 48 hours . Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
This organism is very sensitive to oxygen. It is also requires rumen fluid for growth. All media must be completely reduced. Any oxygen exposure will cause the culture to become nonviable.

This item will only grow on #1703 agar. While very scant growth may be observed on anaerobic blood plates (brucella blood or TSA with 5% sheep blood) after extended incubation, it is not recommended. No growth should occur on the aerobic blood plates.

Always use freshly prepared prereduced media or prereduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions has not been met and oxidation has occurred. Medium should be discarded.

Quality control specifications
Verification method
Whole-genome Sequencing

Supplier来源:BioVector NTCC Inc.
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