pASK90 plasmid vector原核抗体表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989865
- 货 号：pASK90
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pASK90 plasmid vector原核抗体表达质粒载体
Oligonucleotides corresponding to flanking regions of the E. coli ClC gene yadQ (Blattner et al. 1997) were used to PCR-amplify the gene from E. coli genomic DNA. The PCR product was blunt-end ligated into the ZeroBlunt vector and subcloned into pASK90 , downstream from the tetracycline promoter. The sequence of the open reading frames of the products of two independent PCR reactions matched the sequence in the E. coli TIGR database. A hexahistidine tag was added immediately after the initiating methionine codon using PCR mutagenesis, and the resulting construct, pEriC, was used in all experiments.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Oligonucleotides corresponding to flanking regions of the E. coli ClC gene yadQ (Blattner et al. 1997) were used to PCR-amplify the gene from E. coli genomic DNA. The PCR product was blunt-end ligated into the ZeroBlunt vector and subcloned into pASK90 , downstream from the tetracycline promoter. The sequence of the open reading frames of the products of two independent PCR reactions matched the sequence in the E. coli TIGR database. A hexahistidine tag was added immediately after the initiating methionine codon using PCR mutagenesis, and the resulting construct, pEriC, was used in all experiments.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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