pET-44c(+) Vector原核NUS标签表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：pET-44c(+) Vector
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pET-44c(+) Vector原核NUS标签表达载体质粒
The pET-44 vectors are designed for cloning and high-level expression of peptide sequencesfused with the 495 aa Nus•Tag™ protein. Compared to the pET-43.1 series, the pET-44 vectorsencode an additional N-terminal His•Tag. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the COLIDOWN primer (Cat. No. 70845-3). Vector encodedsequence can be completely removed when cloning into the PshA I or Sma I sites (as shownbelow) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
The pET-44 vectors are designed for cloning and high-level expression of peptide sequencesfused with the 495 aa Nus•Tag™ protein. Compared to the pET-43.1 series, the pET-44 vectorsencode an additional N-terminal His•Tag. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the COLIDOWN primer (Cat. No. 70845-3). Vector encodedsequence can be completely removed when cloning into the PshA I or Sma I sites (as shownbelow) by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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