K562-CD19-LUCIFERASE cell line CAR-T荧光稳转细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥989865
- 货 号：K562-CD19-LUCIFERASE
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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K562-CD19-LUCIFERASE cell line CAR-T荧光稳转细胞株
Human Chronic Myelogenous Leukemia Stable Cell Line K562, CD19/Luciferase/EGFP OverexpressedSpecificationsSpeciesHumanCell TypeHuman chronic myeloid leukemia cellOverexpressed GeneCD19-Luciferase-EGFPSize3x10^6 cell/ vialCulture Condition1640+10% FBSGrowth PatternSuspension cultureCell Purity>95%Cell Viability>90%Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.Handling NotesCell Recovery:1. Put on a protective mask and antifreeze gloves, remove the cells from the liquid nitrogen tank, and quickly put them in a 37°C water bath to thaw.2. After the cells are thawed, transfer the cells to the cell chamber through the transfer chamber.3. In the biological safety cabinet, take out a 15mL sterile centrifuge tube, and use a 1mL pipette to add 4mL medium to the 15mL centrifuge tube.4. Use a 75% medical disinfectant alcohol cotton ball to carefully wipe the outer surface of the cell cryopreservation tube that has been thawed in a 37°C water bath for surface disinfection. In the biological safety cabinet, unscrew the cell cryopreservation tube, then use a 1mL pipette to aspirate the cell suspension in the tube, add it dropwise to the 15mL centrifuge tube containing 4mL medium prepared above, and screw the lid of the 15mL centrifuge tube. Tightly, slowly turn upside down 4 times to mix the cell suspension.5. Place the centrifuge tube containing the cell suspension in the centrifuge and set the centrifugation parameters as follows: Centrifuge at 200xg at room temperature (~25°C) for 5 minutes.6. After centrifugation, gently transfer the centrifuge tube to the biological safety cabinet. At this time, white cell clusters can be seen at the bottom of the cytocentrifuge tube. Use an electric aspirator to aspirate the medium supernatant (the cell cluster is relatively loose, be careful not to touch the cell cluster at the bottom).7. Use a 1mL pipette to add 2mL of complete medium, gently pipette 5 times to resuspend the cell pellet, count, and inoculate.8. Place the cells in an incubator for overnight culture.WarningsAvoid multiple freeze/thaw cyclesResearch Use OnlyOur recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.TumorgenicityPositive (In vitro/vivo transformation assay)OncogenicityPositive (In vitro soft agarose assay and life-time studies )
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Human Chronic Myelogenous Leukemia Stable Cell Line K562, CD19/Luciferase/EGFP OverexpressedSpecificationsSpeciesHumanCell TypeHuman chronic myeloid leukemia cellOverexpressed GeneCD19-Luciferase-EGFPSize3x10^6 cell/ vialCulture Condition1640+10% FBSGrowth PatternSuspension cultureCell Purity>95%Cell Viability>90%Mycoplasma TestingThe cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.Handling NotesCell Recovery:1. Put on a protective mask and antifreeze gloves, remove the cells from the liquid nitrogen tank, and quickly put them in a 37°C water bath to thaw.2. After the cells are thawed, transfer the cells to the cell chamber through the transfer chamber.3. In the biological safety cabinet, take out a 15mL sterile centrifuge tube, and use a 1mL pipette to add 4mL medium to the 15mL centrifuge tube.4. Use a 75% medical disinfectant alcohol cotton ball to carefully wipe the outer surface of the cell cryopreservation tube that has been thawed in a 37°C water bath for surface disinfection. In the biological safety cabinet, unscrew the cell cryopreservation tube, then use a 1mL pipette to aspirate the cell suspension in the tube, add it dropwise to the 15mL centrifuge tube containing 4mL medium prepared above, and screw the lid of the 15mL centrifuge tube. Tightly, slowly turn upside down 4 times to mix the cell suspension.5. Place the centrifuge tube containing the cell suspension in the centrifuge and set the centrifugation parameters as follows: Centrifuge at 200xg at room temperature (~25°C) for 5 minutes.6. After centrifugation, gently transfer the centrifuge tube to the biological safety cabinet. At this time, white cell clusters can be seen at the bottom of the cytocentrifuge tube. Use an electric aspirator to aspirate the medium supernatant (the cell cluster is relatively loose, be careful not to touch the cell cluster at the bottom).7. Use a 1mL pipette to add 2mL of complete medium, gently pipette 5 times to resuspend the cell pellet, count, and inoculate.8. Place the cells in an incubator for overnight culture.WarningsAvoid multiple freeze/thaw cyclesResearch Use OnlyOur recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.Quality ControlCultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.TumorgenicityPositive (In vitro/vivo transformation assay)OncogenicityPositive (In vitro soft agarose assay and life-time studies )
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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