pEc-crRNA1 plasmid vector基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pEc-crRNA1 plasmid vector基因编辑质粒载体

encodes E. coli type I-E single spacer array

Vector backboneColE1

Backbone size w/o insert (bp)1688
Total vector size (bp)1852
Vector typeCRISPR, Synthetic Biology
GROWTH IN BACTERIA
Bacterial Resistance(s)Chloramphenicol
Growth Temperature37°C
Growth Strain(s)Top10
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameE. coli type I-E single spacer array
gRNA/shRNA sequenceAATTCTGGCGAATCCTTTAATTAACTGACCAG
PromoterJ23119
CLONING INFORMATION
Cloning methodGibson Cloning
5′ sequencing primerCACCTCTGACTTGAGCGTCG

Supplier来源:BioVector NTCC Inc.
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